지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이 논문의 연구 히스토리 (3)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
The real-time enrichment and detection of pathogens are serious issues and rapidly evolving field of research because of the ability of these pathogens to cause infectious diseases. In general, bacterial detection is accomplished by conventional colony counting or by polymerase chain reaction (PCR) after DNA extraction. As colony counting requires considerable time to cultivate, PCR is an attractivemethod for rapid detection. A small number of pathogens can cause diseases. Hence, a pretreatment process, such as enrichment isessential for detecting bacteria in an actual environment. Thus, in this study, we developed a microfluidic chip capable of performing rapid enrichment of bacteria and the extraction of their genes. A lectin, i.e., Concanavalin A (ConA), which shows binding affinity tothe surface of most bacteria, was coated on the surface of magnetic particles to nonspecifically capture bacteria. It was subsequently con-centrated through magnetic forces in a microfluidic channel. To lyse the captured bacteria, magnetic particles were irradiated by a wave-length of 532nm. The photo-thermal effect on the particles was sufficient for extracting DNA, which was consequently utilized for the identification of bacteria. Our device will help monitor the existence of bacteria in various environmental situations such as water, air, and soil.
목차
등록된 정보가 없습니다.
참고문헌 (7)
참고문헌 신청
H. S. Moon / 2012 / Review of recent progress in micro-systems for the detection and analysis of airborne microorganisms / Anal. Lett. 45(2-3):113~129
H. S. Moon / 2009 / Dielectrophoretic separation of airborne microbes and dust particles using a microfluidic channel for real-time bioaerosol monitoring / Environ. Sci. Technol. 43(15):5857~5863
H. S. Moon / 2010 / Real-time detection of food-borne bacterial adenosine triphosphate (ATP) using dielectrophoretic force and a bioluminescence sensor / Microchim. Acta 170(3-4):283~288
K. Kwon / 2017 / Continuous adsorption and photothermal lysis of airborne bacteria using a gold-nanoparticle-embedded-geometrically activated surface interaction (gold-GASI) chip / Sens. Actuators B 248(1):580~588
J. B. Sumner / 1936 / Identification of hemagglutinin of jack bean with concanavalin A / J. Bacterial. 32(2):227~237
H. S. Moon / 2009 / Dielectrophoretic separation of airborne microbes and dust particles using a microfluidic channel for real-time bioaerosol monitoring / Environ. Sci. Technol. 43(15):5857~5863
H. S. Moon / 2010 / Real-time detection of food-borne bacterial adenosine triphosphate (ATP) using dielectrophoretic force and a bioluminescence sensor / Microchim. Acta 170(3-4):283~288
K. Kwon / 2017 / Continuous adsorption and photothermal lysis of airborne bacteria using a gold-nanoparticle-embedded-geometrically activated surface interaction (gold-GASI) chip / Sens. Actuators B 248(1):580~588
J. B. Sumner / 1936 / Identification of hemagglutinin of jack bean with concanavalin A / J. Bacterial. 32(2):227~237
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Concanavalin A가 처리된 헤링본 구조의 미세유체 칩을 이용한 공기 중 미생물의 연속적 농축 및 용해
한국생산제조학회 학술발표대회 논문집
2016 .11
-
Floating Magnetic Membrane for Rapid Enrichment of Pathogenic Bacteria
BioChip Journal
2021 .01
-
On-chip Capture and Detection of Bacteria with a Controlled Antimicrobial Peptide
한국생물공학회 학술대회
2022 .09
-
Rapid and Sensitive Detection of Pathogen based on Microfluidic Enrichment with Label-Free NanoBiosensing Platform
한국생물공학회 학술대회
2018 .10
-
Touchable 3D hierarchically structured polyaniline nanoweb for capture and detection of pathogenic bacteria
Nano Convergence
2021 .10
-
Methods for Genetic Manipulation of Bacteria
한국생물공학회 학술대회
2017 .04
-
A Rapid Method for Estimation of Cariogenic Bacteria Based on a Stationary Liquid Phase Lab-on-a-chip
BioChip Journal
2018 .01
-
Chitosan-functionalized Magnetic Nanoparticles for Colorimetric Bacteria Detection
한국생물공학회 학술대회
2020 .06
-
Field-effect Transistor Based Portable Microfluidic Device for Real-time Monitoring of Pathogenic Bacteria
한국생물공학회 학술대회
2021 .04
-
New horizon to detect, monitor, and diagnose super-bacteria
한국생물공학회 학술대회
2016 .10
-
Fast Antimicrobial Susceptibility Testing Against Bacteria Using Droplet-based Microfluidic Device
한국생물공학회 학술대회
2021 .04
-
Development of in-situ bacteria detection system suitable for water resources management
대한환경공학회 학술발표논문집
2015 .10
-
저농도 균액 샘플의 농축을 위한 미세유체 기반의 바이오칩
대한기계학회 춘추학술대회
2021 .04
-
Study on treatment of acid mine drainage by nano zero-valent iron synergistic with SRB immobilized particles
Environmental Engineering Research
2021 .10
-
Isolation and Identification of organic waste biodegrading bacteria from soil and waste
대한환경공학회 학술발표논문집
2020 .11
-
Colorimetric Bacteria Detection Method Based on Peroxidase-like Chitosan-Coated Magnetic Nanoparticles
한국생물공학회 학술대회
2023 .10
-
순환종양세포 농축을 위한 3차원 미세유체 칩
한국생산제조학회 학술발표대회 논문집
2015 .04
-
Development of Portable Real-time Fluorescence Monitoring Instrument for Multiplex Detection of Food Poisoning Bacteria
한국생물공학회 학술대회
2021 .10
-
Fully Automated System for Rapid Enrichment and Precise Detection of Enterobacteria Using Magneto-Electrochemical Impedance Measurements
BioChip Journal
2021 .09
-
Fabrication of 3D Printing Based DNA Extraction Microdevice for Foodborne Pathogen Analysis
한국생물공학회 학술대회
2023 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0