지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Quartz crystal microbalance immunosensor has a capacity to perform a label-free and real time detection of a trace amount of analyte through the specific interaction between antibody and antigen. However, immobilization of antibody molecules on the sensor surface is a troublesome procedure for researchers who are not experienced in chemistry. Protein G has a specific affinity to antibody and would serve as a capturing agent for antibody when immobilized on the sensor surface. In this work, we prepared a protein G sensor chip by immobilizing protein G on the surface of quartz crystal microbalance and examined its capability to detect human haptoglobin or human transferrin with unpurified corresponding antiserum. Specific and dose dependent response was observed when the protein G chip was used for detection of antigens after saturated with antiserum. We also verified several advantageous aspects of the protein G chip such as improved flexibility and sensitivity.
목차
등록된 정보가 없습니다.
참고문헌 (14)
참고문헌 신청
A. L. Ghindilis / 1998 / Immunosensors: electrochemical sensing and other engineering approaches / Biosens. Bioelectron 13 : 113 ~ 131
B. kerstrm / 1986 / A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties / J. Biol. Chem 261 : 10240 ~ 10247
C. R. Goward / 1990 / Expression and purification of a truncated recombinant streptococcal protein G / Biochem. J 267 : 171 ~ 177
G. Kronvall / 1979 / Specific absorption of human serum albumin, immunoglobulin A, and immunoglobulin G with selected strains of group A and G streptococci / Infect. Immun 2
H. C. Lu / 2000 / A reusable and specific protein A-coated piezoelectric biosensor for flow injection immunoassay / Biotechnol. Prog 16 : 116 ~ 124
B. kerstrm / 1986 / A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties / J. Biol. Chem 261 : 10240 ~ 10247
C. R. Goward / 1990 / Expression and purification of a truncated recombinant streptococcal protein G / Biochem. J 267 : 171 ~ 177
G. Kronvall / 1979 / Specific absorption of human serum albumin, immunoglobulin A, and immunoglobulin G with selected strains of group A and G streptococci / Infect. Immun 2
H. C. Lu / 2000 / A reusable and specific protein A-coated piezoelectric biosensor for flow injection immunoassay / Biotechnol. Prog 16 : 116 ~ 124
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