지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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This study introduced the development of a strip type disposable enzyme-linked immunosensor platform for the detection of IgG. Strips of the strip sensor were fabricated by using commercial nitrocellulose filter membranes and a housing holder for the strips was manufactured by using a standard injection molding process for a plastic material. An IgG-urease conjugate was prepared and used for the competitive immune-binding with sample IgG. From the enzymatic reaction between the conjugated urease and urea added, ammonia was generated and caused a localized alkaline pH change on the immobilized antibody band which was coated onto the sensor strips. This pH increase subsequently caused a color change of the antibody band in the presence of a pH indicator, phenol red. Used in conjunction with a competitive immunoassay format, the intensity of the color produced is directly linked with the concentration of target analyte, IgG, and specific measurement of IgG in a lateral flow immunoassay format was achieved over the range 100 ppb to 2000 ppb IgG.
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참고문헌 (16)
참고문헌 신청
/ 200809 / Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157 : H7 / ULTRAMICROSCOPY 108 (10) : 1348 ~ 1351
C. Medina-Torres / 2010 / Validation of a commercial enzyme immunoassayfor detection of clostridium difficile toxins in feces ofhorses with acute diarrhea / J. Veteri. Intern
E. Brindle / 2010 / Serum,plasma,and dried blood spot high-sensitivityC-reactive protein enzyme immunoassay forpopulation research / J. Immunological Methods 362 : 112 ~ 120
E. Garet / 2010 / Comparative evaluation of enzyme-linked immunoassay and reference methods for the detection of shellfish hydrophilic toxins in several presentations of sea
K. Janssen / 2010 / Implementation of real-time RTPCR for detection of human metapneumovirus and its comparison with enzyme immunoassay / Archiv. Virology 155 : 207 ~ 215
C. Medina-Torres / 2010 / Validation of a commercial enzyme immunoassayfor detection of clostridium difficile toxins in feces ofhorses with acute diarrhea / J. Veteri. Intern
E. Brindle / 2010 / Serum,plasma,and dried blood spot high-sensitivityC-reactive protein enzyme immunoassay forpopulation research / J. Immunological Methods 362 : 112 ~ 120
E. Garet / 2010 / Comparative evaluation of enzyme-linked immunoassay and reference methods for the detection of shellfish hydrophilic toxins in several presentations of sea
K. Janssen / 2010 / Implementation of real-time RTPCR for detection of human metapneumovirus and its comparison with enzyme immunoassay / Archiv. Virology 155 : 207 ~ 215
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