지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 73 - 79 (7page)
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Background: A Pyrosequencing assay has been used in identification of fungal species such as Candida or Aspergillus and diagnosis of pathogenic bacteria such as Helicobacter pylori but there has been no report on successful isolation and identification of Malassezia yeasts using the pyrosequencing method. Objective: Examine the applicability and plausibility of the pyrosequencing method in identification of the Malassezia species. Methods: At internal transcribed spacer (ITS) sites 1 and 2, three primers were developed using Pyrosequencing Assay Design Software (Biotage AB). Pyrosequencing was performed on 11 standard strains and 83 genomic DNA samples obtained from 66 healthy controls aged from 1 to 80. Results: The eleven Malassezia standard species and 83 genomic DNA samples were successfully identified using the pyrosequencing assay. Conclusion: The pyrosequencing method is a new tool for analysis of Malassezia yeasts, and its precision and rapidity suggests its clinical applicability.
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참고문헌 (22)
참고문헌 신청
Ahn KJ / 1998 / Taxonomy of the genus malassezia / Korean J Med Mycol 3 : 81 ~ 88
Chowdhary A / 2005 / Malassezia furfur in a case of onychomycosis: colonizer or etiologic agent? / Med Mycol 43 : 87 ~ 90
Curvale-Fauchet N / 2004 / Frequency of intravascular catheter colonization by Malassezia spp. in adult patients / Mycoses 47 : 491 ~ 494
Devlin RK / 2006 / Invasive fungal infections caused by Candida and Malassezia species in the neonatal intensive care unit / Adv Neonatal Care 6 : 68 ~ 77
Gaitanis G /
Chowdhary A / 2005 / Malassezia furfur in a case of onychomycosis: colonizer or etiologic agent? / Med Mycol 43 : 87 ~ 90
Curvale-Fauchet N / 2004 / Frequency of intravascular catheter colonization by Malassezia spp. in adult patients / Mycoses 47 : 491 ~ 494
Devlin RK / 2006 / Invasive fungal infections caused by Candida and Malassezia species in the neonatal intensive care unit / Adv Neonatal Care 6 : 68 ~ 77
Gaitanis G /
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