지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 296 - 302 (7page)
이용수
초록· 키워드
오류제보하기
Background: Although vitamin D analogs have been used inthe topical treatment of psoriasis, their mechanisms of actionare not well understand. Calcitriol, the hormonally activevitamin D3 metabolite, has been demonstrated to exertimmunomodulatory effects in the skin by down-regulatingthe expression of Toll-like receptors (TLRs) and proinflammatorycytokines. Objective: We investigated the effects ofcalcitriol on the expression of TLR2, TLR4, antimicrobialpeptide LL-37, and proinflammatory cytokines in culturedhuman keratinocytes. Methods: The mRNA expressionlevels of TLR2, TLR4, tumor necrosis factor α (TNF-α),interleukin (IL)-1β and LL-37 in cultured human keratinocyteswere measured by real-time polymerase chain reaction(PCR) and reverse transcription (RT). Furthermore, wemeasured supernatant TNF-α levels by an enzyme- linkedimmunosorbent assay (ELISA) to confirm the effects of calcitriolon TLR2 and TLR4. Results: As measured by RT-PCRand real-time PCR, calcitriol was found to suppress thelipopolysaccharide- and ultraviolet B radiation-mediatedinduction of expression of TLRs, LL-37 and proinflammatorycytokines such as TNF-α and IL-1β in normal humankeratinocytes. The supernatant TNF-α levels measured byELISA were also suppressed after treatment with calcitriol.
Conclusion: Calcitriol may down- regulate inflammatorystated over-expression of LL-37 and proinflammatory cytokines.
목차
등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Chamorro CI / 2009 / The human antimicrobial peptide LL-37 suppresses apoptosis in keratinocytes / J Invest Dermatol 129 : 937 ~ 944
Cohen-Sfady M / 2005 / Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway / J Immunol 175 : 3594 ~ 3602
Do JE / 2008 / Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease / Rheumatology 47 : 840 ~ 848
Gambichler T / 2008 / Differential mRNA expression of antimicrobial peptides and proteins in atopic dermatitis as compared to psoriasis vulgaris and healthy skin / Int Arch
Khoo AL / 2011 / Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective c
Cohen-Sfady M / 2005 / Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway / J Immunol 175 : 3594 ~ 3602
Do JE / 2008 / Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease / Rheumatology 47 : 840 ~ 848
Gambichler T / 2008 / Differential mRNA expression of antimicrobial peptides and proteins in atopic dermatitis as compared to psoriasis vulgaris and healthy skin / Int Arch
Khoo AL / 2011 / Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective c
이 논문의 저자 정보
최근 본 자료
댓글(0)
0