지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 239 - 245 (7page)
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초록· 키워드
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Background: Neuropathic pain induced by spinal or peripheral nerve injury is very resistant to common pain killers, nerve block, and other pain management approaches. Recently, several studies using stem cells suggested a new way to control the neuropatic pain. In this study, we used the spinal nerve L5 ligation (SNL) model to investigate whether intrathecal rat mesenchymal stem cells (rMSCs) were able to decrease pain behavior, as well as the relationship between rMSCs and reactive oxygen species (ROS).
Methods: Neuropathic pain of the left hind paw was induced by unilateral SNL in Sprague-Dawley rats (n = 10 in each group). Mechanical sensitivity was assessed using Von Frey filaments at 3, 7, 10, 12, 14, 17, and 24 days post-ligation. rMSCs (10 μl, 1 × 105) or phosphate buffer saline (PBS, 10 μl) was injected intrathecally at 7 days post-ligation. Dihydroethidium (DHE), an oxidative fluorescent dye, was used to detect ROS at 24 days post-ligation.
Results: Tight ligation of the L5 spinal nerve induced allodynia in the left hind paw after 3 days post-ligation. ROS expression was increased significantly (P ＜ 0.05) in spinal dorsal horn of L5. Intrathecal rMSCs significantly (P ＜ 0.01) alleviated the allodynia at 10 days after intrathecal injection (17 days post-ligation). Intrathecal rMSCs administration significantly (P ＜ 0.05) reduced ROS expression in the spinal dorsal horn.
Conclusions: These results suggest that rMSCs may modulate neuropathic pain generation through ROS expression after spinal nerve ligation.
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참고문헌 (27)
참고문헌 신청
Chang-Hwan Kim / 2006 / Motor Function Recovery after Adipose Tissue Derived Mesenchym
Conget PA / 1999 / Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells / J Cell Physiol 181 : 67 ~ 73
Gilron I / 2006 / Neuropathic pain : a practical guide for the clinician / CMAJ 175 : 265 ~ 275
Harraz MM / 2008 / SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model / J Clin Invest 118 : 659 ~ 670
Hill RG / 2001 / Molecular basis for the perception of pain / Neuroscientist 7 : 282 ~ 292
Conget PA / 1999 / Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells / J Cell Physiol 181 : 67 ~ 73
Gilron I / 2006 / Neuropathic pain : a practical guide for the clinician / CMAJ 175 : 265 ~ 275
Harraz MM / 2008 / SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model / J Clin Invest 118 : 659 ~ 670
Hill RG / 2001 / Molecular basis for the perception of pain / Neuroscientist 7 : 282 ~ 292
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