개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 446 - 453 (8page)
이용수
초록· 키워드
오류제보하기
Purpose: Long-term use of topical medication is needed for glaucoma treatment. One of the most commonly prescribedclasses of hypotensive agents are prostaglandin analogs (PGs) used as both first-line monotherapy; aswell as in combination therapy with other hypotensive agents. Several side effects of eye drops can be caused bypreservatives. The purpose of this study was to evaluate the effects of PGs with varying concentrations of benzalkoniumchloride (BAC), alternative preservatives, or no preservatives on human conjunctival fibroblast cells.
Methods: Primary human conjunctival fibroblast cells were used in these experiments. Cells were exposed to the followingdrugs: BAC at different concentrations, bimatoprost 0.01% (with BAC 0.02%), latanoprost 0.005% (with BAC0.02%), tafluprost 0.0015% with/without 0.001% BAC and travoprost 0.004% (with 0.001% Polyquad) for 15 and 30minutes. Cell cytotoxicity was evaluated by phase-contrast microscopy to monitor morphological changes of cells,Counting Kit-8 (CCK-8) assay to cell viability, and fluorescent activated cell sorting (FACS) analysis to measureapoptosis.
Results: BAC caused cell shrinkage and detachment from the plate in a dose-dependent manner. Morphologicalchanges were observed in cells treated with bimatoprost 0.01% and latanoprost 0.005%. However, mild cell shrinkagewas noted in cells treated with tafluprost 0.0015%, while a non-toxic effect was noted with travoprost 0.004%and preservative-free tafluprost 0.0015%. CCK-8 assay and FACS analysis showed all groups had a significantlydecreased cell viability and higher apoptosis rate compared with the control group. However, travoprost 0.004%and preservative-free tafluprost 0.0015% showed lower cytotoxicity and apoptosis rate than other drugs.
Conclusions: This in vitro study revealed that BAC-induced cytotoxicity is dose-dependent, although it is importantto emphasize that the clinical significance of toxicity differences observed among the different PGs formulationshas not yet been firmly established. Alternatively preserved or preservative-free glaucoma medications seem to bea reasonable and viable alternative to those preserved with BAC.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Ammar DA / 2010 / Effects of benzalkonium chloride-preserved, polyquad-preserved, and sof- Zia-preserved topical glaucoma medications on human ocular epithelial cells / Adv T
Ammar DA / 2011 / Effects of benzalkonium chloride-and polyquad-preserved combination glaucoma medications on cultured human ocular surface cells / Adv Ther 28 : 501 ~ 510
Baudouin C / 2005 / Allergic reaction to topical eyedrops / Curr Opin Allergy Clin Immunol 5 : 459 ~ 463
Baudouin C / 2007 / In vitro studies of antiglaucomatous prostaglandin analogues : travoprost with and without benzalkonium chloride and preserved latanoprost / Invest Ophtha
Brasnu E / 2008 / In vitro effects of preservative-free tafluprost and preserved latanoprost, travoprost, and bimatoprost in a conjunctival epithelial cell line / Curr Eye Re
Ammar DA / 2011 / Effects of benzalkonium chloride-and polyquad-preserved combination glaucoma medications on cultured human ocular surface cells / Adv Ther 28 : 501 ~ 510
Baudouin C / 2005 / Allergic reaction to topical eyedrops / Curr Opin Allergy Clin Immunol 5 : 459 ~ 463
Baudouin C / 2007 / In vitro studies of antiglaucomatous prostaglandin analogues : travoprost with and without benzalkonium chloride and preserved latanoprost / Invest Ophtha
Brasnu E / 2008 / In vitro effects of preservative-free tafluprost and preserved latanoprost, travoprost, and bimatoprost in a conjunctival epithelial cell line / Curr Eye Re
이 논문의 저자 정보
최근 본 자료
댓글(0)
0