지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 45 - 48 (4page)
이용수
초록· 키워드
오류제보하기
Purpose: To investigate the role of focal adhesion kinase (FAK) in transforming growth factor (TGF)-β-induced myofibroblast transdifferentiation of human Tenon’s fibroblasts.
Methods: Primary cultured human Tenon’s fibroblasts were exposed to TGF-β1 for up to 48 hours. The mRNA levels of FAK, α smooth muscle actin (αSMA), and β-actin were determined by quantitative real time reverse transcription polymerase chain reaction. The protein levels of collagen type I, FAK, phospho-FAK, αSMA, and β-actin were determined by Western immunoblots. After the small interfering RNA targeting FAK (siRNAFAK) molecules were delivered into the cells, the expressions of αSMA proteins were determined by Western immunoblots.
Results: In human Tenon’s fibroblasts, TGF-β1 significantly increased the mRNA and protein expressions of αSMA. However, when the action of FAK was inhibited using siRNAFAK, the TGF-β1-induced expression of αSMA was attenuated.
Conclusions: Our data suggest that FAK may be associated with the TGF-β1-induced transdifferentiation of human Tenon’s fibroblasts to myofibroblasts, which is the essential step of subconjunctival fibrosis.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Brenmoehl J / 2009 / Transforming growth factor-beta 1 induces intestinal myofibroblast differentiation and modulates their migration / World J Gastroenterol 15 : 1431 ~ 144
Chatzizacharias NA / 2010 / The role of focal adhesion kinase in early development / Histol Histopathol 25 : 1039 ~ 1055
Cordeiro MF / 2003 / Technology evaluation: lerdelimumab, Cambridg
Cox BD / 2006 / New concepts regarding focal adhesion kinase promotion of cell migration and proliferation / J Cell Biochem 99 : 35 ~ 52
Desmouliere A / 2004 / The stroma reaction myofibroblast: a key player in the control of tumor cell behavior / Int J Dev Biol 48 : 509 ~ 517
Chatzizacharias NA / 2010 / The role of focal adhesion kinase in early development / Histol Histopathol 25 : 1039 ~ 1055
Cordeiro MF / 2003 / Technology evaluation: lerdelimumab, Cambridg
Cox BD / 2006 / New concepts regarding focal adhesion kinase promotion of cell migration and proliferation / J Cell Biochem 99 : 35 ~ 52
Desmouliere A / 2004 / The stroma reaction myofibroblast: a key player in the control of tumor cell behavior / Int J Dev Biol 48 : 509 ~ 517
이 논문의 저자 정보
최근 본 자료
댓글(0)
0