지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 42 - 47 (6page)
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Purpose: To identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).
Methods: Sprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.
Results: Among a total of 12,731 cDNAs analyzed by mircroarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase Ⅱ inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor;interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin];tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.
Conclusions: We confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associeated with OIR.
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참고문헌 (21)
참고문헌 신청
Budd SJ / 2010 / Increased angiogenic factors associated with peripheral avascular retina and intravitreous neovascularization:a model of retinopathy of prematurity / Arch O
Byfield G / 2009 / The role of supplemental oxygen and JAK/STAT signaling in intravitreous neovascularization in a ROP rat model / Invest Ophthalmol Vis Sci 50 : 3360 ~ 3365
Chan-Ling T / 1992 / Vascular changes and their mechanisms in the feline model of retinopathy of prematurity / Invest Ophthalmol Vis Sci 33 : 2128 ~ 2147
Early Treatment for Retinopathy of Prematurity Cooperative Group / 2003 / Revised indications for the treatment of retinopathy of prematurity:results of the early treatment f
Kim YH / 2005 / Expression of 14-3-3 zeta and interaction with protein kinase C in the rat retina in early diabetes / Diabetologia 48 : 1411 ~ 1415
Byfield G / 2009 / The role of supplemental oxygen and JAK/STAT signaling in intravitreous neovascularization in a ROP rat model / Invest Ophthalmol Vis Sci 50 : 3360 ~ 3365
Chan-Ling T / 1992 / Vascular changes and their mechanisms in the feline model of retinopathy of prematurity / Invest Ophthalmol Vis Sci 33 : 2128 ~ 2147
Early Treatment for Retinopathy of Prematurity Cooperative Group / 2003 / Revised indications for the treatment of retinopathy of prematurity:results of the early treatment f
Kim YH / 2005 / Expression of 14-3-3 zeta and interaction with protein kinase C in the rat retina in early diabetes / Diabetologia 48 : 1411 ~ 1415
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