지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 314 - 344 (31page)
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Purpose: To comparatively analyze the methodological efficacy of the polymerase chain reaction (PCR) assay for herpes simplex virus 1 (HSV) detection in tears.
Methods: This retrospective study reviewed the medical records of 115 patients who were clinically diagnosed with herpes keratitis, and their tear samples were collected for HSV detection. PCR positive rates were analyzed for their dependence on the PCR primers used (conventional PCR primer vs. nested PCR primer), the tear collecting method used (micropipetting vs. collection with schirmer strip), the disease manifestation and the patient’s previous medication history.
Results: HSV DNA was detected in 23 out of 115 (20%) tear samples. The PCR positive rate in tear samples did not differ depending on the PCR primer or tear collection method used. Typical epithelial lesions showed a higher positive rate (31.4%) than atypical epithelial lesions (10.9%). The previous history of the antiviral agent seemed to affect the PCR positive rate.
Conclusions: Although the PCR positive rate was not dependent on the tear collection method or primers, HSV detection in tears using PCR was shown to be a supplementary diagnostic test in typical and atypical herpes epithelitis.
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참고문헌 (19)
참고문헌 신청
Boerman RH / 1989 / Polymerase chain reaction and viral culture techniques to detect HSV in small volumes of cerebrospinal fluid : an experimental mouse encephalitis study /
Choi MY / 1997 / A study on the detection of herpes simplex virus using nested PCR / Korean J Clin Pathol 17 : 764 ~ 771
Crouse CA / 1990 / Detection of herpes viral genomes in normal and diseased corneal epithelium / Curr Eye Res 9 : 569 ~ 581
El-Aal AM / 2006 / Evaluation of herpes simplex detection in corneal scrapings by three molecular methods / Curr Microbiol 52 : 379 ~ 382
Farhatullah S / 2004 / Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings /
Choi MY / 1997 / A study on the detection of herpes simplex virus using nested PCR / Korean J Clin Pathol 17 : 764 ~ 771
Crouse CA / 1990 / Detection of herpes viral genomes in normal and diseased corneal epithelium / Curr Eye Res 9 : 569 ~ 581
El-Aal AM / 2006 / Evaluation of herpes simplex detection in corneal scrapings by three molecular methods / Curr Microbiol 52 : 379 ~ 382
Farhatullah S / 2004 / Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings /
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