지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 373 - 378 (6page)
이용수
초록· 키워드
오류제보하기
ObjectiveTo evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) orbrain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos.
MethodsMature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance mediumsupplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocystformation rate and their cell numbers were assessed.
ResultsThe blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groupswhen compared with the control. However, the blastocyst formation rate and the total cell count was significantlyhigher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8±11.5 vs. 52.3%,38.0±6.8; P<0.05, respectively).
ConclusionSupplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos,but supplementation of rmGM-CSF did not.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Aflalo ED / 2007 / EGF increases expression and activity of PAs in preimplantation rat embryos and their implantation rate / Reprod Biol Endocrinol 5 : 4 ~
Anderson RA / 2010 / Brainderived neurotrophic factor is a regulator of human oocyte maturation and early embryo development / Fertil Steril 93 : 1394 ~ 1406
Barbacid M / 1994 / The Trk family of neurotrophin receptors / J Neurobiol 25 : 1386 ~ 1403
Caceres-Cortes J / 1994 / Product of the steel locus suppresses apoptosis in hemopoietic cells: comparison with pathways activated by granulocyte macrophage colony-stimulatin
Dissen GA / 1995 / Expression of neurotrophins and their receptors in the mammalian ovary is developmentally regulated: changes at the time of folliculogenesis / Endocrinolo
Anderson RA / 2010 / Brainderived neurotrophic factor is a regulator of human oocyte maturation and early embryo development / Fertil Steril 93 : 1394 ~ 1406
Barbacid M / 1994 / The Trk family of neurotrophin receptors / J Neurobiol 25 : 1386 ~ 1403
Caceres-Cortes J / 1994 / Product of the steel locus suppresses apoptosis in hemopoietic cells: comparison with pathways activated by granulocyte macrophage colony-stimulatin
Dissen GA / 1995 / Expression of neurotrophins and their receptors in the mammalian ovary is developmentally regulated: changes at the time of folliculogenesis / Endocrinolo
이 논문의 저자 정보
최근 본 자료
댓글(0)
0