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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 14 - 19 (6page)
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Introduction: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli ) to oral pathogens through signal exchange.
Materials and Methods: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (Δ K-12), and Scleropages aureus (S.
aureus ) were used. A single culture consisting of Hep2, HT29, WT K-12, and Δ K-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and Δ K-12, WT K-12 and S. aureus , Δ K-12 and S. aureus , and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus . Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR).
Results: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus . Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-κB was higher in the mixed culture of Hep2 with Δ K-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2.
Conclusion: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.
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참고문헌 (21)
참고문헌 신청
Bamler AJ / 1994 / Salmonellaty phimurium loci involved in survival within macrophages / Infect Immun 62 : 1623 ~ 1630
Braiteh F / 2007 / Cryptogenic invasive Klebsiella pneumoniae liver abscess syndrome / Int J Infect Dis 11 : 16 ~ 22
Frisken KW / 1990 / The incidence of periodontopathic microorganisms in young children / Oral Microbiol Immunol 5 : 43 ~ 45
Fujihara A / 2002 / Detection of tmRNA-mediated trans-translation products in Bacillus subtilis / Genes Cells 7 : 343 ~ 350
Genco RJ / 1988 / The origin of periodontal infections / Adv Dent Res 2 : 245 ~ 259
Braiteh F / 2007 / Cryptogenic invasive Klebsiella pneumoniae liver abscess syndrome / Int J Infect Dis 11 : 16 ~ 22
Frisken KW / 1990 / The incidence of periodontopathic microorganisms in young children / Oral Microbiol Immunol 5 : 43 ~ 45
Fujihara A / 2002 / Detection of tmRNA-mediated trans-translation products in Bacillus subtilis / Genes Cells 7 : 343 ~ 350
Genco RJ / 1988 / The origin of periodontal infections / Adv Dent Res 2 : 245 ~ 259
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