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학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

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논문 기본 정보

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학술저널
저자정보
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대한구강악안면외과학회 대한구강악안면외과학회지 대한구강악안면외과학회지 제36권 제3호
발행연도
2010.1
수록면
186 - 196 (11page)

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Introduction: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. Materials and Methods: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. Results: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. Conclusion: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.
#Human dental tissue-derived stem cells #Dental pulp stem cells #DPSCs) #Dental follicle cells #DFCs) #Stem cells from root apical papilla #SCAP) #Mesenchymal stem cells #MSCs)

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참고문헌 (27)

참고문헌 신청
Almeida-Porada G / 2001 / Adult stem cell plasticity and methods of detection / Rev Clin Exp Hematol 5 : 26 ~ 41 google schola Avilion AA / 2003 / Multipotent cell lineages in early mouse development depend on SOX2 function / Genes Dev 17 : 126 ~ 140 google schola Boyer LA / 2005 / Core transcriptional regulatory circuitry in human embryonic stem cells / Cell 122 : 947 ~ 956 google schola Carlin R / 2006 / Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC google schola Chambers I / 2003 / Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells / Cell 113 : 643 ~ 655 google schola

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