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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제2호
- 발행연도
- 2015.1
- 수록면
- 162 - 173 (12page)
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초록· 키워드
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The cellular fatty acid composition is important for metabolic plasticity in Rhodobacter sphaeroides. We explored the effects of changing the cellular ratio of unsaturated fatty acids (UFAs) to saturated fatty acids (SFAs) in R. sphaeroides by overexpressing several key fatty acid biosynthetic enzymes through the use of expression plasmid pRK415. Bacteria containing the plasmid pRKfabI1 with the fabI1 gene that encodes enoyl-acyl carrier protein (ACP) reductase showed a reduction in the cellular UFA to SFA ratio from 4 (80% UFA) to 2 (65% UFA) and had decreased membrane fluidity and reduced cell growth. Additionally, the ratio of UFA to SFA of the chromatophore vesicles from pRKfabI1-containing cells was similarly lowered, and the cell had decreased levels of light-harvesting complexes, but no change in intracytoplasmic membrane (ICM) content or photosynthetic (PS) gene expression. Both inhibition of enoyl- ACP reductase with diazaborine and addition of exogenous UFA restored membrane fluidity, cell growth, and the UFA to SFA ratio to wild-type levels in this strain. R. sphaeroides containing the pRKfabB plasmid with the fabB gene that encodes the enzyme β-ketoacyl-ACP synthase I exhibited an increased UFA to SFA ratio from 4 (80% UFA) to 9 (90% UFA), but showed no change in membrane fluidity or growth rate relative to control cells. Thus, membrane fluidity in R. sphaeroides remains fairly unchanged when membrane UFA levels are between 80% and 90%, whereas membrane fluidity, cell growth, and cellular composition are affected when UFA levels are below 80%.
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참고문헌 (22)
참고문헌 신청
Aguilar PS / 2006 / Control of fatty acid desaturation: a mechanism conserved from bacteria to humans / Mol. Microbiol 62 : 1507 ~ 1514
Aktas M / 2009 / In vitro characterization of the enzyme properties of the phospholipid N-methyltransferase PmtA from Agrobacterium tumefaciens / J. Bacteriol 191 : 2033 ~ 20
Aricha B / 2004 / Differences in membrane fluidity and fatty acid composition between phenotypic variants of Streptococcus pneumoniae / J. Bacteriol 186 : 4638 ~ 4644
Arondel V / 1993 / Isolation and functional expression in Escherichia coli of a gene encoding phosphatidylethanolamine methyltransferase (EC 2.1.1.17)from Rhodobacter sphaero
Baldock C / 1996 / A mechanism of drug action revealed by structural studies of enoyl reductase / Science 274 : 2107 ~ 2110
Aktas M / 2009 / In vitro characterization of the enzyme properties of the phospholipid N-methyltransferase PmtA from Agrobacterium tumefaciens / J. Bacteriol 191 : 2033 ~ 20
Aricha B / 2004 / Differences in membrane fluidity and fatty acid composition between phenotypic variants of Streptococcus pneumoniae / J. Bacteriol 186 : 4638 ~ 4644
Arondel V / 1993 / Isolation and functional expression in Escherichia coli of a gene encoding phosphatidylethanolamine methyltransferase (EC 2.1.1.17)from Rhodobacter sphaero
Baldock C / 1996 / A mechanism of drug action revealed by structural studies of enoyl reductase / Science 274 : 2107 ~ 2110
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