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학술저널
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제4호
발행연도
2014.1
수록면
475 - 482 (8page)

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The metabolic pathway of eugenol degradation by thermophilic Geobacillus sp. AY 946034strain was analyzed based on the lack of data about eugenol degradation by thermophiles. TLC, GC-MS, and biotransformation with resting cells showed that eugenol was oxidizedthrough coniferyl alcohol, and ferulic and vanillic acids to protocatechuic acid before thearomatic ring was cleaved. The cell-free extract of Geobacillus sp. AY 946034 strain grown oneugenol showed a high activity of eugenol hydroxylase, feruloyl-CoA synthetase, vanillate-Odemethylase,and protocatechuate 3,4-dioxygenase. The key enzyme, protocatechuate 3,4-dioxygenase, which plays a crucial role in the degradation of various aromatic compounds,was purified 135-fold to homogeneity with a 34% overall recovery from Geobacillus sp. AY946034. The relative molecular mass of the native enzyme was about 450 ± 10 kDa and wascomposed of the non-identical subunits. The pH and temperature optima for enzyme activitywere 8 and 60oC, respectively. The half-life of protocatechuate 3,4-dioxygenase at the optimumtemperature was 50 min.

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