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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제6호
- 발행연도
- 2013.1
- 수록면
- 794 - 801 (8page)
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This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp.
Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v):0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone;initial pH 7.0; incubation time 72 h; 30oC; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at 60oC and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0–30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0–30.0%(v/v) ethanol at 30oC for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 μmol/ml reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase.
These properties suggested the potential of the crude enzyme for being applied in the animal feed industry,xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.
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참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] -
Ahmed, S. / 2009 / Molecular cloning of fungal xylanases: An overview / Appl. Microbiol. Biotechnol 84 : 19 ~ 35
Akpinar, O. / 2009 / Xylooligosaccharide production from lignocellulosic wastes with Trichoderma longibrachiatum xylanase / J. Food Agric. Environ 7 : 70 ~ 74
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Cai, H. Y. / 2011 / A novel thermoacidophilic family 10 xylanase from Penicillium pinophilum C1 / Process Biochem 46 : 2341 ~ 2346
Ahmed, S. / 2009 / Molecular cloning of fungal xylanases: An overview / Appl. Microbiol. Biotechnol 84 : 19 ~ 35
Akpinar, O. / 2009 / Xylooligosaccharide production from lignocellulosic wastes with Trichoderma longibrachiatum xylanase / J. Food Agric. Environ 7 : 70 ~ 74
Bradford, M. M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 7
Cai, H. Y. / 2011 / A novel thermoacidophilic family 10 xylanase from Penicillium pinophilum C1 / Process Biochem 46 : 2341 ~ 2346
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