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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제6호
- 발행연도
- 2013.1
- 수록면
- 872 - 877 (6page)
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초록· 키워드
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In a previous study, we isolated and reported a second species of the Saccharophagus genus, Saccharophagus sp.
strain Myt-1. In the present study, an alginate lyase gene (algMytC) from the genomic DNA of Myt-1 was cloned and characterized. The DNA sequence fragment obtained contained an open reading frame of 1,032 bp that encoded a protein of 343 amino acids with an estimated molecular mass of 37.6 kDa and a pI of 6.60. The deduced protein,AlgMytC, had the conserved amino acid sequences (RTELREM, QIH, YFKAGVYNQ) of the polysaccharide lyase family 7. A BLAST homology search indicated that AlgMytC shared an amino acid sequence identity of 95.9% with alg7A of S. degradans 2-40. The cloned and purified AlgMytC protein showed optimal activity at 40oC, and retained more than 90% of its total activity even after treatment at 25oC for 24 h. AlgMytC was very alkaliphilic with an optimal pH of 9.0, and more than 90% of its activity was retained in the pH range 8.5-10.0.
Moreover, AlgMytC was stable over a wide pH range. The activity of AlgMytC was also stable in the presence of various detergents.
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참고문헌 (24)
참고문헌 신청
Akiyama, H. / 1992 / Effect of depolymerized alginates on the growth of bifidobacteria / Biosci. Biotechnol. Biochem 56 : 355 ~ 356
An, Q. D. / 2009 / Alginate-deriving oligosaccharide production by alginase from newly isolated Flavobacterium sp. LXA and its potential application in protection against pat
Andrykovitch, G. / 1988 / Isolation of a new polysaccharide-digesting bacterium from a salt marsh / Appl. Environ. Microbiol 54 : 1061 ~ 1062
Calumpong, H. P. / 1999 / Population and alginate yield and quality assessment of four Sargassum species in Negros Island, central Philippines Hydrobiologia 398/399 : 211 ~ 2
Ekborg, N. A. / 2005 / Saccharophagus degradans gen. nov., sp. nov., a versatile marine decomposer of complex polysaccharides / Int. J. Syst. Evol. Microbiol 55 : 1545 ~ 1549
An, Q. D. / 2009 / Alginate-deriving oligosaccharide production by alginase from newly isolated Flavobacterium sp. LXA and its potential application in protection against pat
Andrykovitch, G. / 1988 / Isolation of a new polysaccharide-digesting bacterium from a salt marsh / Appl. Environ. Microbiol 54 : 1061 ~ 1062
Calumpong, H. P. / 1999 / Population and alginate yield and quality assessment of four Sargassum species in Negros Island, central Philippines Hydrobiologia 398/399 : 211 ~ 2
Ekborg, N. A. / 2005 / Saccharophagus degradans gen. nov., sp. nov., a versatile marine decomposer of complex polysaccharides / Int. J. Syst. Evol. Microbiol 55 : 1545 ~ 1549
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