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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제4호
- 발행연도
- 2013.1
- 수록면
- 489 - 498 (10page)
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초록· 키워드
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A new Bacillus strain degrading starch, named Bacillus sp. UEB-S, was isolated from a southern Tunisian area.
Amylase production using solid-state fermentation on millet, an inexpensive and available agro-resource, was investigated. Response surface methodology was applied to establish the relationship between enzyme production and four variables: inoculum size, moisture-to-millet ratio,temperature, and fermentation duration. The maximum enzyme activity recovered was 680 U/g of dry substrate when using 1.38 × 109 CFU/g as inoculation level, 5.6:1 (ml/g)as moisture ratio (86%), for 4 days of cultivation at 37oC,which was in perfect agreement with the predicted model value. Amylase was purified by Q-Sepharose anion-exchange and Sephacryl S-200 gel filtration chromatography with a 14-fold increase in specific activity. Its molecular mass was estimated at 130 kDa. The enzyme showed maximal activity at pH 5 and 70oC, and efficiently hydrolyzed starch to yield glucose and maltose as end products. The enzyme proved its efficiency for digesting raw cereal below gelatinization temperature and, hence, its potentiality to be used in industrial processes.
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참고문헌 (23)
참고문헌 신청
Adeyanju, M. M. / 2007 / A thermostable extracellular α-amylase from Bacillus licheniformis isolated from cassava steep water / Biotechnology 6 : 473 ~ 480
American Association of Cereal Chemists(AACC) / 2000 / Approved Methods of the AACC, In Methods 46-30, 30-10, 44-19, 08-01 / The American Association of Cereal Chemists
Anto, H. / 2006 / Alpha amylase production by Bacillus cereus MTCC 1305 using solid-state fermentation / Food Technol. Biotechnol 44 : 241 ~ 245
Arikan, B. / 2008 / Highly thermostable, thermophilic, alkaline, SDS and chelating resistant amylase from thermophilic Bacillus isolate A3-15 / Bioresour. Technol 99 : 3071 ~
Asgher, M. / 2007 / A thermostable amylase from a moderately thermophilic Bacillus subtilis strain for starch processing / J. Food Eng 79 : 950 ~ 955
American Association of Cereal Chemists(AACC) / 2000 / Approved Methods of the AACC, In Methods 46-30, 30-10, 44-19, 08-01 / The American Association of Cereal Chemists
Anto, H. / 2006 / Alpha amylase production by Bacillus cereus MTCC 1305 using solid-state fermentation / Food Technol. Biotechnol 44 : 241 ~ 245
Arikan, B. / 2008 / Highly thermostable, thermophilic, alkaline, SDS and chelating resistant amylase from thermophilic Bacillus isolate A3-15 / Bioresour. Technol 99 : 3071 ~
Asgher, M. / 2007 / A thermostable amylase from a moderately thermophilic Bacillus subtilis strain for starch processing / J. Food Eng 79 : 950 ~ 955
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