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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제4호
- 발행연도
- 2013.1
- 수록면
- 459 - 466 (8page)
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초록· 키워드
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The lactic acid bacterium Streptococcus thermophilus is widely used as a starter culture for the production of dairy products. Whole-genome sequencing is expected to utilize the genetic basis behind the metabolic functioning of lactic acid bacterium (LAB), for development of their use in biotechnological and probiotic applications. We sequenced the whole genome of Streptococcus thermophilus MTCC 5461, the strain isolated from a curd source, by 454 GS-FLX titanium and Ion Torrent PGM. We performed comparative genome analysis using the local BLAST and RDP for 16S rDNA comparison and by the RAST server for functional comparison against the published genome sequence of Streptococcus thermophilus CNRZ 1066. The whole genome size of S. thermophilus MTCC 5461 is of 1.73Mb size with a GC content of 39.3%. Streptococcal virulence-related genes are either inactivated or absent in the strain. The genome possesses coding sequences for features important for a probiotic organism such as adhesion, acid tolerance, bacteriocin production, and lactose utilization, which was found to be conserved among the strains MTCC 5461 and CNRZ 1066. Biochemical analysis revealed the utilization of 17 sugars by the bacterium,where the presence of genes encoding enzymes involved in metabolism for 16 of these 17 sugars were confirmed in the genome. This study supports the facts that the strain MTCC 5461 is nonpathogenic and harbors essential features that can be exploited for its probiotic potential.
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참고문헌 (23)
참고문헌 신청
Altermann, E. / 2005 / Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM / Proc. Natl. Acad. Sci. USA 102 : 3906 ~ 3912
Bolotin, A.. / 2004 / Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus / Nat. Biotechnol 22 : 1554 ~ 1558
Burton, J. P. / 2006 / Safety assessment of the oral cavity probiotic Streptococcus salivarius K12 / Appl. Environ. Microbiol 72 : 3050 ~ 3053
Coenye, T. / 2003 / Extracting phylogenetic information from whole-genome sequencing projects: The lactic acid bacteria as a test case / Microbiology 149 : 3507 ~ 3517
Dave, R. I. / 1991 / Standardizing conditions for pilot scale production and storage of buffalo milk dahi using selected strains of Streptococcus thermophilus / Gujarat Agricultural Un
Bolotin, A.. / 2004 / Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus / Nat. Biotechnol 22 : 1554 ~ 1558
Burton, J. P. / 2006 / Safety assessment of the oral cavity probiotic Streptococcus salivarius K12 / Appl. Environ. Microbiol 72 : 3050 ~ 3053
Coenye, T. / 2003 / Extracting phylogenetic information from whole-genome sequencing projects: The lactic acid bacteria as a test case / Microbiology 149 : 3507 ~ 3517
Dave, R. I. / 1991 / Standardizing conditions for pilot scale production and storage of buffalo milk dahi using selected strains of Streptococcus thermophilus / Gujarat Agricultural Un
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