지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,629 - 1,635 (7page)
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초록· 키워드
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Previously, we demonstrated that the erythropoietin receptor (EpoR) is present on fibroblasts, where it regulates focal contact. Here, we assessed whether this action of EpoR is involved in the reduced cell adhesion observed in colonocytes exposed to Clostridium difficile toxin A. EpoR was present and functionally active in cells of the human colonic epithelial cell line HT29 and epithelial cells of human colon tissues. Toxin A significantly decreased activating phosphorylations of EpoR and its downstream signaling molecules JAK-2 (Janus kinase 2) and STAT5(signal transducer and activator of transcription 5). In vitro kinase assays confirmed that toxin A inhibited JAK 2kinase activity. Pharmacological inhibition of JAK2 (with AG490) abrogated activating phosphorylations of EpoR and also decreased focal contacts in association with inactivation of paxillin, an essential focal adhesion molecule.
In addition, AG490 treatment significantly decreased expression of occludin (a tight junction molecule) and tight junction levels. Taken together, these data suggest that inhibition of JAK2 by toxin A in colonocytes causes inactivation of EpoR, thereby enhancing the inhibition of focal contact formation and loss of tight junctions known to be associated with the enzymatic activity of toxin A.
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참고문헌 (22)
참고문헌 신청
Alscher, K. T. / 2001 / Enteral feeding decreases gut apoptosis, permeability, andlung inflammation during murine endotoxemia / Am. J. Physiol.Gastrointest. Liver Physiol 281
Choi, Hye-Ryung / 2012 / Protein Tyrosine Phosphatase Profiling Analysis of HIB-1B Cells during Brown Adipogenesis / Journal of Microbiology and Biotechnology 22 (7) : 1029 ~
Di Bona, D. / 2006 / Oxidative stress inhibits IFNalpha- induced antiviral gene expression by blocking the JAKSTAT pathway / J. Hepatol 45 : 271 ~ 279
Farrell, F. / 2004 / The erythropoietin receptor and its expression in tumor cells and other tissues / Oncologist 5 : 18 ~ 30
Huang, L. J. / 2001 / The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor / Mol. Cell 8 : 1327 ~ 1
Choi, Hye-Ryung / 2012 / Protein Tyrosine Phosphatase Profiling Analysis of HIB-1B Cells during Brown Adipogenesis / Journal of Microbiology and Biotechnology 22 (7) : 1029 ~
Di Bona, D. / 2006 / Oxidative stress inhibits IFNalpha- induced antiviral gene expression by blocking the JAKSTAT pathway / J. Hepatol 45 : 271 ~ 279
Farrell, F. / 2004 / The erythropoietin receptor and its expression in tumor cells and other tissues / Oncologist 5 : 18 ~ 30
Huang, L. J. / 2001 / The N-terminal domain of Janus kinase 2 is required for Golgi processing and cell surface expression of erythropoietin receptor / Mol. Cell 8 : 1327 ~ 1
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