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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,782 - 1,789 (8page)
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초록· 키워드
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We have developed a novel type of polymer nanogel loaded with anticancer drug based on bio-derived poly(γ-glutamic acid) (γ-PGA). γ-PGA is a highly anionic polymer that is synthesized naturally by microbial species, most prominently in various bacilli, and has been shown to have excellent biocompatibility. Thiolated γ-PGA was synthesized by covalent coupling between the carboxyl groups of γ-PGA and the primary amine group of cysteamine. Doxorubicin (Dox)-loaded γ-PGA nanogels were fabricated using the following steps: (1) an ionic nanocomplex was formed between thiolated γ-PGA as the negative charge component, and Dox as the positive charge component; (2) addition of poly(ethylene glycol)(PEG) induced hydrogen-bond interactions between thiol groups of thiolated γ-PGA and hydroxyl groups of PEG,resulting in the nanocomplex; and (3) disulfide crosslinked γ-PGA nanogels were fabricated by ultrasonication.
The average size and surface charge of Dox-loaded disulfide cross-linked γ-PGA nanogels in aqueous solution were 136.3 ± 37.6 nm and -32.5 ± 5.3 mV, respectively. The loading amount of Dox was approximately 38.7 μg per mg of γ-PGA nanogel. The Dox-loaded disulfide cross-linked γ-PGA nanogels showed controlled drug release behavior in the presence of reducing agents, glutathione (GSH) (1-10 mM). Through fluorescence microscopy and FACS, the cellular uptake of γ-PGA nanogels into breast cancer cells (MCF-7) was analyzed. The cytotoxic effect was evaluated using the MTT assay and was determined to be dependent on both the concentration and treatment time of γ-PGA nanogels. The bio-derived γ-PGA nanogels are expected to be a well-designed delivery carrier for controlled drug delivery applications.
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참고문헌 (22)
참고문헌 신청
Anderson, M. E. / 1998 / Glutathione: An overview of biosynthesis and modulation / Chem. Biol. Interact 112 : 1 ~ 14
Bae, K. H. / 2008 / Synthesis, characterization, and intracellular delivery of reducible heparin nanogels for apoptotic cell death / Biomaterials 29 (23) : 3376 ~ 3383
Bae, Sun-Ryang / 2010 / Effects of Ultra High Molecular Weight Poly-γ-glutamic Acid from Bacillus subtilis (chungkookjang) on Corneal Wound Healing / Journal of Microbiology
Bernkop-Schnurch, A. / 2005 / Thiomers: A new generation of mucoadhesive polymers / Adv. Drug Deliver. Rev 57 : 1569 ~ 1582
Bravo-Osuna, I. / 2007 / Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surface / Int. J. Pharm 340 : 173 ~ 181
Bae, K. H. / 2008 / Synthesis, characterization, and intracellular delivery of reducible heparin nanogels for apoptotic cell death / Biomaterials 29 (23) : 3376 ~ 3383
Bae, Sun-Ryang / 2010 / Effects of Ultra High Molecular Weight Poly-γ-glutamic Acid from Bacillus subtilis (chungkookjang) on Corneal Wound Healing / Journal of Microbiology
Bernkop-Schnurch, A. / 2005 / Thiomers: A new generation of mucoadhesive polymers / Adv. Drug Deliver. Rev 57 : 1569 ~ 1582
Bravo-Osuna, I. / 2007 / Characterization of chitosan thiolation and application to thiol quantification onto nanoparticle surface / Int. J. Pharm 340 : 173 ~ 181
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