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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,644 - 1,652 (9page)
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초록· 키워드
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Iron plays a key role in host-pathogen interactions.
Microbial pathogens require iron for survival and virulence,whereas mammalian hosts sequester and withhold iron as a means of nutritional immunity. We previously identified two paralogous genes, CFT1 and CFT2, which encode homologs of a fungal iron permease, Cft1 and Cft2,respectively, in the human fungal pathogen Cryptococcus neoformans. Cft1 was shown to play a role in the highaffinity reductive iron uptake system, and was required for transferrin utilization and full virulence in mammalian hosts. However, no role of Cft2 has been suggested yet.
Here, we identified the third gene, CFT3, that produces an additional fungal iron permease homolog in C. neoformans,and we also generated the cft3 mutant for functional characterization. We aimed to reveal distinct functions of Cft1, Cft2 and Cft3 by analyzing phenotypes of the mutants lacking CFT1, CFT2 and CFT3, respectively. The endogenous promoter of CFT1, CFT2 and CFT3 was replaced with the inducible GAL7 promoter in the wildtype strain or in the cft1 mutant for gain-of-function analysis. Using these strains, we were able to find that CFT2 is required for growth in low-iron conditions in the absence of CFT1 and that overexpression of CFT2compensates for deficiency of the cft1 mutant in iron uptake and various cellular stress conditions. However,unlike CFT2, no clear phenotypic characteristic of the cft3mutant and the strain overexpressing CFT3 was observed.
Overall, our data suggested a redundant role of Cft2in the high-affinity iron uptake and stress responses in C. neoformans.
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참고문헌 (24)
참고문헌 신청
Askwith, C. / 1994 / The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake / Cell 76 : 403 ~ 410
Choi, J. / 2012 / Regulated expression of cyclic AMP-dependent protein kinase A reveals an influence on cell size and the secretion of virulence factors in Cryptococcus neofo
Dancis, A. / 1994 / Molecular characterization of a copper transport protein in S. cerevisiae: An unexpected role for copper in iron transport / Cell 76 : 393 ~ 402
Davidson, R. C. / 2002 / A PCR-based strategy to generate integrative targeting alleles with large regions of homology / Microbiology 148 : 2607 ~ 2615
Hentze, M. W. / 2004 / Balancing acts: Molecular control of mammalian iron metabolism / Cell 117 : 285 ~ 297
Choi, J. / 2012 / Regulated expression of cyclic AMP-dependent protein kinase A reveals an influence on cell size and the secretion of virulence factors in Cryptococcus neofo
Dancis, A. / 1994 / Molecular characterization of a copper transport protein in S. cerevisiae: An unexpected role for copper in iron transport / Cell 76 : 393 ~ 402
Davidson, R. C. / 2002 / A PCR-based strategy to generate integrative targeting alleles with large regions of homology / Microbiology 148 : 2607 ~ 2615
Hentze, M. W. / 2004 / Balancing acts: Molecular control of mammalian iron metabolism / Cell 117 : 285 ~ 297
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