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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,653 - 1,658 (6page)
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초록· 키워드
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We have reported previously that a recombinant Escherichia coli co-expresses aminolevulinic acid (ALA) synthase, an NADP-dependent malic enzyme, and a dicarboxylate transporter-produced heme, an iron-chelated porphyrin,in a succinate-containing complex medium. To develop an industrially plausible process, a chemically defined medium was formulated based on M9 minimal medium. Heme synthesis was enhanced by adding sodium bicarbonate,which strengthened the C4 metabolism required for the precursor metabolite, although a pH change discouraged cell growth. Increasing the medium pH buffering capacity (100mM phosphate buffer) and adding sodium bicarbonate enabled the recombinant E. coli to produce heme at rates 60% greater than those in M9 minimal medium. Adding growth factors (1 mg/l thiamin, 0.01 mg/l biotin, 5 mg/l nicotinic acid, 1 mg/l pantothenic acid, and 1.4 mg/l cobalamin) also induced positive heme production effects at levels twice of heme production in M9-based medium.
Porphyrin derivatives and heme were found in the chemically defined medium, and their presence was confirmed by liquid chromatography/mass spectroscopy (LC/MS). The formulated medium allowed for the production of 0.6 μM heme, 29 μM ALA, 0.07 μM coproporphyrin I, 0.21 μM coproporphyrin III, and 0.23 μM uroporphyrin in a 3 L pH-controlled culture.
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참고문헌 (16)
참고문헌 신청
Afonso, S. / 1998 / Porphyrin-induced protein structural alternations of heme enzymes. II: Protection of 5-aminolevulinic acid dehydratase and porphobilinogen deaminase from
Bu, W. / 2003 / Simultaneous determination of six urinary porphyrins using liquid chromatography-tandem mass spectrometry / J. Chromatogr. B Analyt. Technol. Biomed. Life Sci
Chen, W. / 1994 / 5-Aminolevulinic acid synthesis in Escherichia coli requiresexpression of hemA / J. Bacteriol 176 : 2743 ~ 2746
Duin, E. C. / 1997 / [2Fe-2S] to [4Fe- 4S] cluster conversion in Escherichia coli biotin synthase / Biochemistry 36 : 11811 ~ 11820
Kwon, S. J. / 2003 / High-level production of porphyrins in metabolicallyengineered Escherichia coli: Systematic extension of a pathwayassembled from overexpressed genes invo
Bu, W. / 2003 / Simultaneous determination of six urinary porphyrins using liquid chromatography-tandem mass spectrometry / J. Chromatogr. B Analyt. Technol. Biomed. Life Sci
Chen, W. / 1994 / 5-Aminolevulinic acid synthesis in Escherichia coli requiresexpression of hemA / J. Bacteriol 176 : 2743 ~ 2746
Duin, E. C. / 1997 / [2Fe-2S] to [4Fe- 4S] cluster conversion in Escherichia coli biotin synthase / Biochemistry 36 : 11811 ~ 11820
Kwon, S. J. / 2003 / High-level production of porphyrins in metabolicallyengineered Escherichia coli: Systematic extension of a pathwayassembled from overexpressed genes invo
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