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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제5호
- 발행연도
- 2011.1
- 수록면
- 477 - 482 (6page)
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There are accumulating evidences suggesting that connexin (Cx), a gap junction channel-forming protein, acts as a growth suppressor in various cancer cells, and this effect is attributeed to the gap junction-mediated intercellular communication (GJIC). In order to characterize the relationship between the growth-arresting activity of Cx26 and its cytoplasmic localizations after expression,we linked a nuclear export signal (NES) sequence to Cx26cDNA before transfecting into a rat breast cancer cell line.
A confocal fluorescent microscopic observation revealed that the insertion of NES minimized the nuclear expression of Cx26, and increased its cytoplasmic expression, including plasma membrane junctions. Total cell counting and BrdUrd-labeling experiments showed that the growth of the breast cancer cells was inhibited by 74% upon transfection of Cx26-NES, whereas only 9% inhibition was observed with only Cx26 cDNA.
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참고문헌 (24)
참고문헌 신청
Bond, S. L / 1994 / Transfection of C6 glioma cells with connexin32: The effects of expression of a nonendogenous gap junction protein / Cell Growth Differ 5 : 179 ~ 186
Bruzzone, R / 1996 / Connections with connexins: The molecular basis of direct intercellular signaling / Eur. J. Biochem 238 : 1 ~ 27
Cascio, M / 1995 / Physical characterization of gap junction membrane connexons (hemi-channels) isolated from rat liver / J. Biol. Chem 270 : 18643 ~ 18648
Chen, S. C / 1995 / Connexin43 reverses the phenotype of transformed cells and alters their expression of cyclin/cyclin-dependent kinases / Cell Growth Differ 6 : 681 ~ 690
Dang, X / 2003 / The carboxy-tail of connexin-43 localizes to the nucleus and inhibits cell growth / Mol. Cell Biochem 242 : 35 ~ 38
Bruzzone, R / 1996 / Connections with connexins: The molecular basis of direct intercellular signaling / Eur. J. Biochem 238 : 1 ~ 27
Cascio, M / 1995 / Physical characterization of gap junction membrane connexons (hemi-channels) isolated from rat liver / J. Biol. Chem 270 : 18643 ~ 18648
Chen, S. C / 1995 / Connexin43 reverses the phenotype of transformed cells and alters their expression of cyclin/cyclin-dependent kinases / Cell Growth Differ 6 : 681 ~ 690
Dang, X / 2003 / The carboxy-tail of connexin-43 localizes to the nucleus and inhibits cell growth / Mol. Cell Biochem 242 : 35 ~ 38
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