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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제3호
- 발행연도
- 2011.1
- 수록면
- 256 - 262 (7page)
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초록· 키워드
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A 20.9-kDa metalloprotease was isolated from dried fruiting bodies of the wild basidiomycete mushroom Lepista nuda. The N-terminal amino acid sequence of the protease was seen to be ATFVLTAATNTLFTA, thus displaying no similarity with the sequences of previously reported metalloproteases. The protease was purified using a procedure that entailed ion-exchange chromatography on CM-Cellulose, Q-Sepharose, and Mono S, and FPLC-gel filtration on Superdex 75. The protease functioned at an optimum pH of 7.0 and an optimum temperature of 50℃.
It was also noted that the protease demonstrated a proteolytic activity of 1,756 U/mg toward casein. The Km of the purified protease toward casein was 6.36 mg/ml at a pH of 7.0 and with a temperature of 37℃, whereas the Vmax was 9.11 μg m^(-1) min^(-1) . The activity of the protease was adversely affected by EDTA-2Na, suggesting that it is a metalloprotease. PMSF, EGTA, aprotinin, and leupeptin exerted no striking inhibitory effect. The activity of the protease was enhanced by Fe²+ , but was curtailed by Cd²+,Cu²+, Hg²+, Pb²+, Zn²+, and Fe³+ ions. The protease also exhibited inhibitory activity against HIV-1 reverse transcriptase with an IC_(50) value of 4.00 μM. The IC_(50)values toward hepatoma Hep G2 and leukemia L1210cells in vitro were 4.99 μM and 3.67 μM, respectively.
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참고문헌 (24)
참고문헌 신청
Adil, A / 1998 / Alkaline proteases / A review. Bioresour. Technol 64 : 175 ~ 183
Barros, L / 2008 / Chemical composition and biological properties of Portuguese wild mushrooms. A comprehensive study / J. Agric. Food Chem 56 : 3856 ~ 3862
Bottcher, M / 1997 / HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction / Nucleic Acids Res 25 : 1709 ~ 1714
Burton, K. S / 1993 / Purification and characterization of a serine proteinase from senescent sporophores of the commercial mushroom Agaricus bisporus / J. Gen. Microbiol 139
Dulger, B / 2002 / Antimicrobial activity of the macrofungus Lepista nuda / Fitoterapia 73 : 695 ~ 697
Barros, L / 2008 / Chemical composition and biological properties of Portuguese wild mushrooms. A comprehensive study / J. Agric. Food Chem 56 : 3856 ~ 3862
Bottcher, M / 1997 / HIV-1 protease inhibits its homologous reverse transcriptase by protein-protein interaction / Nucleic Acids Res 25 : 1709 ~ 1714
Burton, K. S / 1993 / Purification and characterization of a serine proteinase from senescent sporophores of the commercial mushroom Agaricus bisporus / J. Gen. Microbiol 139
Dulger, B / 2002 / Antimicrobial activity of the macrofungus Lepista nuda / Fitoterapia 73 : 695 ~ 697
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