지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제3호
- 발행연도
- 2011.1
- 수록면
- 317 - 322 (6page)
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초록· 키워드
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A monoterpenoid compound, benzylideneacetone (BZA),is identified from bacterial metabolites synthesized by an entomopathogenic bacterium, Xenorhabdus nematophila.
It inhibits phospholipase A₂ of target insects to shut down biosynthesis of various eicosanoids, which play significant roles in insect immunity. This study discovered another novel activity of BZA that directly inhibited phenoloxidase (PO) activity required for immune-associated melanization.
When it was injected into larvae of Plutella xylostella, it suppressed PO activity in the plasma by inhibiting its activation from inactive proPO. However, BZA did not influence on gene expression of PO, which was analyzed by RT-PCR using gene-specific primers designed from a partial cDNA sequence of PO of the P. xylostella identified in this study. To test a direct inhibitory activity of BZA against PO, the activated PO of P. xylostella was prepared from the hemolymph collected from the larvae challenged by bacteria. When the activated PO was incubated in vitro with BZA, it was inhibited in a dose-dependent manner. The inhibition of PO by BZA was recovered by addition of increasing amounts of substrate, L-3,4-dihydroxyphenylalanine.
Three other known bacterial metabolites containing a benzene propane core structure synthesized by X. nematophila also inhibited the PO enzyme activity. However, modification of the core structure by hydroxylation of BZA lost its strong inhibitory activity against the activated PO.
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참고문헌 (23)
참고문헌 신청
Akhurst,R.J / 1980 / Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically associated with the insect pathogenic nematodes Neoaplectana and Hete
Balsinde, J / 2002 / Phospholipase A2 regulation of arachidonic acid mobilization / FEBS Lett 531 : 2 ~ 6
Bidla, G / 2007 / Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger / J. Cell Sci 120 : 1209 ~ 1215
Boemare,N / 2002 / Biology, taxonomy and systematic of Photorhabdus and Xenorhabdus in Entomopathogenic Nematology / CABI Publishing : 35 ~ 56
Brunet,P.C.J / 1980 / The metabolism of the aromatic amino acids concerned in the cross-linking of insect cuticle / Insect Biochem 10 : 467 ~ 500
Balsinde, J / 2002 / Phospholipase A2 regulation of arachidonic acid mobilization / FEBS Lett 531 : 2 ~ 6
Bidla, G / 2007 / Crystal cell rupture after injury in Drosophila requires the JNK pathway, small GTPases and the TNF homolog Eiger / J. Cell Sci 120 : 1209 ~ 1215
Boemare,N / 2002 / Biology, taxonomy and systematic of Photorhabdus and Xenorhabdus in Entomopathogenic Nematology / CABI Publishing : 35 ~ 56
Brunet,P.C.J / 1980 / The metabolism of the aromatic amino acids concerned in the cross-linking of insect cuticle / Insect Biochem 10 : 467 ~ 500
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