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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제3호
- 발행연도
- 2011.1
- 수록면
- 236 - 242 (7page)
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초록· 키워드
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A psychrotrophic bacterium, Arthrobacter sp. ON14, isolated from Antarctica, was shown to exhibit a high β-galactosidase activity at a low temperature. A genomic library of ON14was constructed and screened for β-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as the substrate. Two different β-galactosidase genes, named as galA, galB, were found in ON14. Computational analyses of the genes revealed that the encoded protein GalA belongs to family 2 of glycosyl hydrolysases and is a cold-active protein, whereas GalB belongs to family 42 of glycosyl hydrolysases and is a mesophilic protein. Reverse transcription analyses revealed that the expression of galA is highly induced at a low temperature (4℃) and repressed at a high temperature (28℃) when lactose is used as the sole carbon source.
Conversely, the expression of galB is inhibited at a low temperature and induced at a high temperature. The purified GalA showed its peak activity at 15℃ and pH 8.
The mineral ions Na+, K+, Mg²+, and Mn²+ were identified as enzyme activators, whereas Ca²+ had no influence on the enzyme activity. An enzyme stability assay revealed that the activity of GalA is significantly decreased when it is incubated at 45℃ for 2 h, and all its activity is lost when it is incubated at 50℃.
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참고문헌 (23)
참고문헌 신청
Bradford,M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72:248~254
Brahamsha, B / 1987 / Complementation of a trpE deletion in Escherichia coli by Spirochaeta aurantia DNA encoding anthranilate synthetase component I activity / J. Bacteriol 169:3764~3769
Coker, J / 2003 / Biochemical characterization of a {beta}-galactosidase with a low temperature optimum obtained from an antarctic Arthrobacter isolate / J. Bacteriol 185:5473~5482
Coombs, J / 1999 / Biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA / Appl. Environ. Microbiol 65:5443~5450
D’amico, S / 2006 / Psychrophilic microorganisms: Challenges for life / EMBO Rep 7:385~389
Brahamsha, B / 1987 / Complementation of a trpE deletion in Escherichia coli by Spirochaeta aurantia DNA encoding anthranilate synthetase component I activity / J. Bacteriol 169:3764~3769
Coker, J / 2003 / Biochemical characterization of a {beta}-galactosidase with a low temperature optimum obtained from an antarctic Arthrobacter isolate / J. Bacteriol 185:5473~5482
Coombs, J / 1999 / Biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium Carnobacterium piscicola BA / Appl. Environ. Microbiol 65:5443~5450
D’amico, S / 2006 / Psychrophilic microorganisms: Challenges for life / EMBO Rep 7:385~389
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