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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제10호
- 발행연도
- 2010.1
- 수록면
- 1,440 - 1,445 (6page)
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초록· 키워드
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Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy,was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 were 8.0, 30oC, and 175 rpm,respectively. In addition, low concentrations of glucose were found to inhibit the degradation of DBP, whereas high concentrations of glucose increased its degradation.
Meanwhile, a substrate utilization test showed that JDC-11 was also able to utilize other phthalates. The major metabolites of DBP degradation were identified as monobutyl phthalate and phthalic acid by gas chromatographymass spectrometry, allowing speculation on the tentative metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11. Using a set of new degenerate primers, a partial sequence of the 3,4-phthalate dioxygenase gene was obtained from JDC-11. Moreover, a sequence analysis revealed that the phthalate dioxygenase gene of JDC-11was highly homologous to the large subunit of the phthalate dioxygenase from Rhodococcus coprophilus strain G9.
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참고문헌 (23)
참고문헌 신청
Chang, B. V / 2004 / Biodegradation of phthalate esters by two bacteria strains / Chemosphere 55 : 533 ~ 538
Chang, H. K / 1998 / Novel organization of the genes for phthalate degradation from Burkholderia cepacia DBO1 / J. Bacteriol 180 : 6529 ~ 6537
Chao, W. L / 2006 / Degradation of dibutyl-phthalate by soil bacteria / Chemosphere 63 : 1377 ~ 1383
Choi, K. Y / 2005 / Molecular and biochemical analysis of phthalate and terephthalate degradation by Rhodococcus sp. strain DK17 / FEMS Microbiol. Lett 252 : 207 ~ 213
Eaton, R. W / 2001 / Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B / J. Bacteriol 183 : 3689 ~ 3703
Chang, H. K / 1998 / Novel organization of the genes for phthalate degradation from Burkholderia cepacia DBO1 / J. Bacteriol 180 : 6529 ~ 6537
Chao, W. L / 2006 / Degradation of dibutyl-phthalate by soil bacteria / Chemosphere 63 : 1377 ~ 1383
Choi, K. Y / 2005 / Molecular and biochemical analysis of phthalate and terephthalate degradation by Rhodococcus sp. strain DK17 / FEMS Microbiol. Lett 252 : 207 ~ 213
Eaton, R. W / 2001 / Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B / J. Bacteriol 183 : 3689 ~ 3703
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