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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제5호
- 발행연도
- 2010.1
- 수록면
- 917 - 924 (8page)
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초록· 키워드
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The practicability of transgenic tobacco engineered to express bacterial native mercuric reductase (MerA), responsible for the transport of Hg2+ ions into the cell and their reduction to elemental mercury (Hg0), without any codon modification,for phytoremediation of mercury pollution was evaluated.
Transgenic tobacco plants reduce mercury ions to the metallic form; take up metallic mercury through their roots;and evolve the less toxic elemental mercury. Transformed tobacco produced a large amount of merA protein in leaves and showed a relatively higher resistance phenotype to HgCl2 than wild type. Results suggest that the integrated merA gene, encoding mercuric reductase, a key enzyme of the bacterial mer operon, was stably integrated into the tobacco genome and translated to active MerA, which catalyzes the bioconversion of toxic Hg2+ to the least toxic elemental Hg0, and suggest that MerA is capable of reducing the Hg2+, probably via NADPH as an electron donor. The transgenic tobacco expressing merA volatilized significantly more mercury than wild-type plants. This is first time we are reporting the expression of a bacterial native merA gene via the nuclear genome of Nicotiana tabacum, and enhanced mercury volatilization from tobacco transgenics.
The study clearly indicates that transgenic tobacco plants are reasonable candidates for the remediation of mercurycontaminated areas.
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참고문헌 (23)
참고문헌 신청
Baya, A. M / 1986 / Coincident plasmid and antimicrobial resistance in marine bacterial isolates from polluted and unpolluted Atlantic Ocean samples / Appl. Environ. Microbio
Birnboim, H. C / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Bizily, S. P / 1999 / Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana plants confers resistance to organomercurials / Proc. Natl. Acad. Sci. U.
Brown, N. L / 1986 / The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: Further evidence for mer genes, which enhance the activi
Carty, A. J / 1979 / The chemistry of mercury in biological systems in The Bioqeochemistry of Mercury in the Elsevier Biomedical : 433 ~ 479
Birnboim, H. C / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Bizily, S. P / 1999 / Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana plants confers resistance to organomercurials / Proc. Natl. Acad. Sci. U.
Brown, N. L / 1986 / The nucleotide sequence of the mercuric resistance operons of plasmid R100 and transposon Tn501: Further evidence for mer genes, which enhance the activi
Carty, A. J / 1979 / The chemistry of mercury in biological systems in The Bioqeochemistry of Mercury in the Elsevier Biomedical : 433 ~ 479
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