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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 1,989 - 1,996 (8page)
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초록· 키워드
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Ice-binding proteins (IBPs) can inhibit ice recrystallization (IR), a major cause of cell death during cryopreservation. IBPs are hypothesized to improve cell viability after cryopreservation by alleviating the cryoinjury caused by IR. In our previous studies, we showed that supplementation of the freezing medium with the recombinant IBP of the Arctic yeast Glaciozyma sp. (designated as LeIBP) could reduce post-thaw hemolysis of human red blood cells and increase the survival of cryopreserved diatoms. Here, we showed that LeIBP could improve the viability of cryopreserved mammalian cells. Human cervical cancer cells (HeLa), mouse fibroblasts (NIH/3T3), human preosteoblasts (MC3T3-E1), Chinese hamster ovary cells (CHO-K1), and human keratinocytes (HaCaT) were evaluated. These mammalian cells were frozen in dimethyl sulfoxide (DMSO)/fetal bovine serum (FBS) solution with or without 0.1 mg/ml LeIBP at a cooling rate of -1°C/min in a -80°C freezer overnight. The minimum effective concentration (0.1 mg/ml) of LeIBP was determined, based on the viability of HeLa cells after treatment with LeIBP during cryopreservation and the IR inhibition assay results. The post-thaw viability of mammalian cells was examined. In all cases, cell viability was significantly enhanced by more than 10% by LeIBP supplementation in 5% DMSO/5% FBS: viability increased by 20% for HeLa cells, 28% for NIH/3T3 cells, 21% for MC3T3-E1, 10% for CHO-K1, and 20% for HaCaT. Furthermore, addition of LeIBP reduced the concentrations of toxic DMSO and FBS down to 5%. Therefore, we demonstrated that LeIBP can increase the viability of cryopreserved mammalian cells by inhibiting IR.
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참고문헌 (24)
참고문헌 신청
Amir G / 2004 / Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein III / Cryobiology 48 : 273 ~ 282
Bagis H / 2008 / Cryogenic effect of antifreeze protein on transgenic mouse ovaries and the production of live offspring by orthotopic transplantation of cryopreserved mouse
Chaytor JL / 2012 / Inhibiting ice recrystallization and optimization of cell viability after cryopreservation / Glycobiology 22 : 123 ~ 133
Dinsmore J / 1996 / Embryonic stem cells differentiated in vitro as a novel source of cells for transplantation / Cell Transplant 5 : 131 ~ 143
Do H / 2014 / Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1 / Acta
Bagis H / 2008 / Cryogenic effect of antifreeze protein on transgenic mouse ovaries and the production of live offspring by orthotopic transplantation of cryopreserved mouse
Chaytor JL / 2012 / Inhibiting ice recrystallization and optimization of cell viability after cryopreservation / Glycobiology 22 : 123 ~ 133
Dinsmore J / 1996 / Embryonic stem cells differentiated in vitro as a novel source of cells for transplantation / Cell Transplant 5 : 131 ~ 143
Do H / 2014 / Structure-based characterization and antifreeze properties of a hyperactive ice-binding protein from the Antarctic bacterium Flavobacterium frigoris PS1 / Acta
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