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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제7호
- 발행연도
- 2014.1
- 수록면
- 925 - 935 (11page)
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초록· 키워드
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A cold-adapted carbohydrate esterase, CEST, belonging to the carbohydrate esterase family 6, was cloned from Microbulbifer thermotolerans DAU221. CEST was composed of 307 amino acids with the first 22 serving as a secretion signal peptide. The calculated molecular mass and isoelectric point of the mature enzyme were 31,244 Da and pH 5.89, respectively. The catalytic triad consisted of residues Ser37, Glu192, and His281 in the conserved regions: GQSNMXG, QGEX(D/N), and DXXH. The three-dimensional structure of CEST revealed that CEST belongs to the α/β-class of protein consisted of a central six-stranded β-sheet flanked by eight α-helices. The recombinant CEST was purified by His-tag affinity chromatography and the characterization showed its optimal temperature and pH were 15°C and 8.0, respectively. Specifically, CEST maintained up to 70% of its enzyme activity when preincubated at 50°C or 60°C for 6 h, and 89% of its enzyme activity when preincubated at 70°C for 1h . The results suggest CEST belongs to group 3 of the cold-adapted enzymes. The enzyme activity was increased by Na+ and Mg2+ ions but was strongly inhibited by Cu+ and Hg2+ ions, at all ion concentrations. Using p-nitrophenyl acetate as a substrate, the enzyme had a Km of 0.278 mM and a kcat of 1.9 s-1. Site-directed mutagenesis indicated that the catalytic triad (Ser37, Glu192, and His281) and Asp278 were essential for the enzyme activity.
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참고문헌 (23)
참고문헌 신청
Al Khudary R / 2010 / A cold-adapted esterase of a novel marine isolate, Pseudoalteromonas arctica : gene cloning, enzyme purification and characterization / Extremophiles 14
Alalouf O / 2011 / A new family of carbohydrate esterases is represented by a GSDL hydrolase/acetylxylan esterase from Geobacillus stearothermophilus / J. Biol. Chem 86 : 419
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
Aurilia V / 2007 / The psychrophilic bacterium Pseudoalteromonas halosplanktis TAC125 possesses a gene coding for a cold-adapted feruloyl esterase activity that shares homolo
Ay F / 2011 / Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1 / Protein Expr. Purif 80 : 74 ~ 79
Alalouf O / 2011 / A new family of carbohydrate esterases is represented by a GSDL hydrolase/acetylxylan esterase from Geobacillus stearothermophilus / J. Biol. Chem 86 : 419
Altschul SF / 1997 / Gapped BLAST and PSI-BLAST: a new generation of protein database search programs / Nucleic Acids Res 25 : 3389 ~ 3402
Aurilia V / 2007 / The psychrophilic bacterium Pseudoalteromonas halosplanktis TAC125 possesses a gene coding for a cold-adapted feruloyl esterase activity that shares homolo
Ay F / 2011 / Cloning, purification and characterization of a thermostable carboxylesterase from Anoxybacillus sp. PDF1 / Protein Expr. Purif 80 : 74 ~ 79
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