지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제5호
- 발행연도
- 2014.1
- 수록면
- 619 - 628 (10page)
이용수
초록· 키워드
오류제보하기
To identify lipase LipA (PFL_0617) from Pseudomonas protegens Pf-5, a lipA deletion mutant (Pf0617) and a complementary strain (Pf0617lipA) were constructed, and their effects on the lipase production were examined. Pf0617 remarkably decreased its whole-cell lipase activity, whereas Pf0617lipA made its whole-cell lipase activity not only restore to wild-type level but also get a further increment. However, the deletion and overexpression of lipA did not affect the extracellular lipase activity. In addition, the unbroken whole cells of these strains were able to catalyze the hydrolysis of membrane-permeable p-nitrophenyl esters, but could not hydrolyze the membrane-impermeable olive oil. These results confirmed that LipA was an intracellular lipase and Pf-5 could also be used as a natural whole-cell biocatalyst. To evaluate the potential of Pf-5 as a whole-cell biocatalyst and separately characterize the whole-cell LipA, the properties of the whole-cell lipases from Pf-5 and Top10lipA were characterized. The results demonstrated that both Pf-5 and Top10lipA exhibited high tolerance to alkaline condition, high temperature, heavy metal ions, surfactants, and organic solvents. Taken together, lipA can realize functional expression in E. coli Top10, and Pf-5 and Top10lipA as whole-cell biocatalysts may have enormous potential in applications.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Angkawidjaja C / 2006 / Family I.3 lipase: bacterial lipases secreted by the type I secretion system / Cell Mol. Life Sci 63 : 2804 ~ 2817
Arpigny JL / 1999 / Bacterial lipolytic enzymes: classification and properties / Biochem. J 343 : 177 ~ 183
Busto E / 2006 / Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase / Nat. Protoc 1 : 2061 ~ 2067
Chakraborty K / 2009 / Purification and biochemical characterization of an extracellular lipase from Pseudomonas fluorescens MTCC 2421 / J. Agric. Food Chem 57 : 3859 ~ 3866
Grbavcic S / 2011 / Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives:compatibility study with de
Arpigny JL / 1999 / Bacterial lipolytic enzymes: classification and properties / Biochem. J 343 : 177 ~ 183
Busto E / 2006 / Kinetic resolution of 4-chloro-2-(1-hydroxyalkyl)pyridines using Pseudomonas cepacia lipase / Nat. Protoc 1 : 2061 ~ 2067
Chakraborty K / 2009 / Purification and biochemical characterization of an extracellular lipase from Pseudomonas fluorescens MTCC 2421 / J. Agric. Food Chem 57 : 3859 ~ 3866
Grbavcic S / 2011 / Production of lipase and protease from an indigenous Pseudomonas aeruginosa strain and their evaluation as detergent additives:compatibility study with de
이 논문의 저자 정보
최근 본 자료
댓글(0)
0