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자료유형
학술저널
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제2호
발행연도
2014.1
수록면
168 - 176 (9page)

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The morphology of filamentous fungi closely correlates with the productivity in submergedculture. Using itaconic acid (IA) production by Aspergillus terreus as a research model, thequantitative relationship between the growth form of A. terreus and IA production wasinvestigated. IA fermentation was scaled up from shake flasks to a 7 L stirred tank bioreactorbased on the quantitative relationship. Our results demonstrated the following: (1) Threemorphologies of A. terreus were formed by changing the inoculum level and shape of the flask. (2) Investigation of the effects of the three morphologies on broth rheology and IA productionrevealed the higher yield of IA on dry cell weight (DCW, IA/DCW) and yield of glucose onDCW (consumed glucose/DCW) were achieved during clump growth of A. terreus. (3) Byvarying the KH2PO4 concentration and culture temperature, the relationships between clumpdiameter and IA production were established, demonstrating that the yield of IA on DCW(R2 = 0.9809) and yield of glucose on DCW (R2 = 0.9421) were closely correlated with clumpdiameter. The optimum clump diameter range for higher IA production was 0.40–0.50 mm. (4)When the clump diameter was controlled at 0.45 mm by manipulating the mechanical stress ina 7 L fermentor, the yield of IA on DCW and yield of glucose on DCW were increased by 25.1%and 16.3%, respectively. The results presented in this study provide a potential approach forfurther enhancement of metabolite production by filamentous fungi.

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