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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제2호
- 발행연도
- 2014.1
- 수록면
- 254 - 263 (10page)
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초록· 키워드
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Enteromorpha polysaccharides (EP) extracted from green algae have displayed a wide varietyof biological activities. However, their high molecular weight leads to a high viscosity and lowsolubility, and therefore, greatly restrains their application. To solve this problem, bacteriafrom the surface of Enteromorpha were screened, and an Alteromonas macleodii strain B7 wasfound to be able to decrease the molecular weight of EP in culture media. Proteins harvestedfrom the supernatant of the A. macleodii B7 culture were subjected to native gel electrophoresis,and a band corresponding to the Enteromorpha polysaccharide lyase (EPL) was detected byactivity staining. The enzyme identity was subsequently confirmed by MALDI-TOF/TOFmass spectrometry as the putative α-amylase reported in A. macleodii ATCC 27126. Theamylase gene (amySTU) from A. macleodii B7 was cloned into Escherichia coli, resulting in highlevelexpression of the recombinant enzyme with EP-degrading activity. AmySTU was foundto be cold-adapted; however, its optimal enzyme activity was detected at 40oC. The α-amylasewas highly stable over a broad pH range (5.5-10) with the optimal pH at 7.5-8.0. The highestenzyme activity was detected when NaCl concentration was 2%, which dropped by 50% whenthe NaCl concentration was increased to 16%, showing an excellent nature of halotolerance.
Furthermore, the amylase activity was not significantly affected by tested surfactants or thepresence of some organic solvents. Therefore, the A. macleodii strain B7 and its α-amylase canbe useful in lowering EP molecular weight and in starch processing.
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참고문헌 (22)
참고문헌 신청
Ben Abdelmalek-Khedher, Imen / 2008 / Purification, Characterization, and Partial Primary Sequence of a Major- Maltotriose-producing α-Amylase, ScAmy43, from Sclerotinia scl
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Ch evolot L / 1999 / Further d ata on t he structure of brown seaweed fucans: relationships with anticoagulant activity / Carbohydr. Res 319 : 154 ~ 165
Chattopadhyay K / 2007 / Sulphated p olysaccharides f rom Indian samples of Enteromorpha compressa (Ulvales, Chlorophyta):isolation and structural features / Food Chem 104 :
Chen M / 2005 / Studies on antimutagenic and the free radical scavenging effect of polysaccharide from Gracilaria lemaneiformis / Food Sci 26 : 219 ~ 222
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Ch evolot L / 1999 / Further d ata on t he structure of brown seaweed fucans: relationships with anticoagulant activity / Carbohydr. Res 319 : 154 ~ 165
Chattopadhyay K / 2007 / Sulphated p olysaccharides f rom Indian samples of Enteromorpha compressa (Ulvales, Chlorophyta):isolation and structural features / Food Chem 104 :
Chen M / 2005 / Studies on antimutagenic and the free radical scavenging effect of polysaccharide from Gracilaria lemaneiformis / Food Sci 26 : 219 ~ 222
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