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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제2호
- 발행연도
- 2014.1
- 수록면
- 245 - 253 (9page)
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초록· 키워드
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A fibrinolytic enzyme was produced by an edible mushroom of Pleurotus ostreatus usingsubmerged culture fermentation. The enzyme was purified from the culture supernatant byapplying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobicinteraction, and gel filtration chromatographies. The enzyme was purified by a 147-fold, witha yield of 7.54%. The molecular masses of the enzyme an determined by gel filtration and SDSPAGEwere 13.6 and 18.2 kDa, respectively. The isoelectric point of the enzyme was 8.52. Ithydrolyzed fibrinogen by cleaving the α and β chains of fibrinogen followed by the γ chains,and also activated plasminogen into plasmin. The enzyme was optimally active at 45°C andpH 7.4. The enzyme activity was completely inhibited by EDTA, whereas protease inhibitorsof TPCK, SBTI, PMSF, aprotinin and pepstatin showed no inhibition on its activity. The partialamino acid sequences of the enzyme as determined by Q-TOF2 were ATFVGCSATR,GGTLIHESSHFTR, and YTTWFGTFVTSR. These sequences showed a high degree ofhomology with those of metallo-endopeptidases from P. ostreatus and Armillaria mellea. Thepurified enzyme can also be applied as a natural agent for oral fibrinolytic therapy orprevention of thrombosis.
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참고문헌 (22)
참고문헌 신청
Andrews P. / 1965 / The gel-filtration behaviour of proteins related to their molecular weights over a wide range / Biochem. J 96 : 596 ~ 606
Astrup T / 1952 / The fibrin plate method for estimating fibrinolytic activity / Arch. Biochem. Biophys 40 : 346 ~ 351
Choi NS / 2009 / Purification and characterization of a novel thermoacid stable fibrinolytic enzyme from Staphylococcus sp. strain AJ isolated from Korean salt-fermented Anch
Cui L / 2008 / A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom / World J. Microbiol. Biotechnol 24 : 483 ~ 489
Dohmae N / 1995 / Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies / Biosci. Biotechnol. Biochem 59 : 2074 ~ 2080
Astrup T / 1952 / The fibrin plate method for estimating fibrinolytic activity / Arch. Biochem. Biophys 40 : 346 ~ 351
Choi NS / 2009 / Purification and characterization of a novel thermoacid stable fibrinolytic enzyme from Staphylococcus sp. strain AJ isolated from Korean salt-fermented Anch
Cui L / 2008 / A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom / World J. Microbiol. Biotechnol 24 : 483 ~ 489
Dohmae N / 1995 / Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies / Biosci. Biotechnol. Biochem 59 : 2074 ~ 2080
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