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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제12호
- 발행연도
- 2018.1
- 수록면
- 1,999 - 2,008 (10page)
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The compound 2,5-furandicarboxylic acid (FDCA), an important bio-based monomer for the production of various polymers, can be obtained from 5-hydroxymethylfurfural (HMF). However, efficient production of FDCA from HMF via biocatalysis has not been well studied. In this study, we report the identification of key genes that are involved in FDCA synthesis and then the engineering of Raoultella ornithinolytica BF60 for biocatalytic oxidation of HMF to FDCA using its resting cells. Specifically, previously unknown candidate genes, adhP3 and alkR, which were responsible for the reduction of HMF to the undesired product 2,5- bis(hydroxymethyl)furan (HMF alcohol), were identified by transcriptomic analysis. Combinatorial deletion of these two genes resulted in 85.7% reduction in HMF alcohol formation and 23.7% improvement in FDCA production (242.0 mM). Subsequently, an aldehyde dehydrogenase, AldH, which was responsible for the oxidation of the intermediate 5-formyl-2-furoic acid (FFA) to FDCA, was identified and characterized. Finally, FDCA production was further improved by overexpressing AldH, resulting in a 96.2% yield of 264.7 mM FDCA. Importantly, the identification of these key genes not only contributes to our understanding of the FDCA synthesis pathway in R. ornithinolytica BF60 but also allows for improved FDCA production efficiency. Moreover, this work is likely to provide a valuable reference for producing other furanic chemica.
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참고문헌 (44)
참고문헌 신청
Zhou C-H / 2011 / Catalytic conversion of lignocellulosic biomass to fine chemicals and fuels / Chem. Soc. Rev 40:5588~5617
Teong SP / 2014 / Hydroxymethylfurfural production from bioresources: past, present and future / Green Chem 16:2015~2026
Mika LT / 2018 / Catalytic Conversion of Carbohydrates to Initial Platform Chemicals:Chemistry and Sustainability / Chem. Rev 118:505~613
Sousa AF / 2015 / Biobased polyesters and other polymers from 2,5-furandicarboxylic acid: a tribute to furan excellency / Polym. Chem 6:5961~5983
Vilela C / 2014 / The quest for sustainable polyesters–insights into the future / Polym. Chem 5:3119~3141
Teong SP / 2014 / Hydroxymethylfurfural production from bioresources: past, present and future / Green Chem 16:2015~2026
Mika LT / 2018 / Catalytic Conversion of Carbohydrates to Initial Platform Chemicals:Chemistry and Sustainability / Chem. Rev 118:505~613
Sousa AF / 2015 / Biobased polyesters and other polymers from 2,5-furandicarboxylic acid: a tribute to furan excellency / Polym. Chem 6:5961~5983
Vilela C / 2014 / The quest for sustainable polyesters–insights into the future / Polym. Chem 5:3119~3141
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