지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제3호
- 발행연도
- 2017.1
- 수록면
- 507 - 513 (7page)
이용수
초록· 키워드
오류제보하기
Bacillus subtilis spores can be used for protein display to engineer protein properties. This method overcomes viability and protein-folding concerns associated with traditional protein display methods. Spores remain viable under extreme conditions and the genotype/ phenotype connection remains intact. In addition, the natural sporulation process eliminates protein-folding concerns that are coupled to the target protein traveling through cell membranes. Furthermore, ATP-dependent chaperones are present to assist in protein folding. CotA was optimized as a whole-cell biocatalyst immobilized in an inert matrix of the spore. In general, proteins that are immobilized have advantages in biocatalysis. For example, the protein can be easily removed from the reaction and it is more stable. The aim is to improve the pH stability using spore display. The maximum activity of CotA is between pH 4 and 5 for the substrate ABTS (ABTS = diammonium 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate). However, the activity dramatically decreases at pH 4. The activity is not significantly altered at pH 5. A library of approximately 3,000 clones was screened. A E498G variant was identified to have a half-life of inactivation (t 1/2) at pH 4 that was 24.8 times greater compared with wt- CotA. In a previous investigation, a CotA library was screened for organic solvent resistance and a T480A mutant was found. Consequently, T480A/E498G-CotA was constructed and the t 1/2 was 62.1 times greater than wt-CotA. Finally, E498G-CotA and T480A/E498G-CotA yielded 3.7- and 5.3-fold more product than did wt-CotA after recycling the biocatalyst seven times over 42 h.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Amstutz P / 2001 / In vitro display technologies: novel developments and applications / Curr. Opin. Biotechnol 12 : 400 ~ 405
Bershtein S / 2008 / Advances in laboratory evolution of enzymes / Curr. Opin. Chem. Biol 12 : 151 ~ 158
Bessette PH / 1999 / Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm / Proc. Natl. Acad. Sci. USA 96 : 13703 ~ 13708
Boa W / 1993 / A laccase associated with lignification in loblolly pine xylem / Science 260 : 672 ~ 674
Claus H / 2004 / Laccases: structure, reactions, distribution / Micron 35 : 93 ~ 96
Bershtein S / 2008 / Advances in laboratory evolution of enzymes / Curr. Opin. Chem. Biol 12 : 151 ~ 158
Bessette PH / 1999 / Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm / Proc. Natl. Acad. Sci. USA 96 : 13703 ~ 13708
Boa W / 1993 / A laccase associated with lignification in loblolly pine xylem / Science 260 : 672 ~ 674
Claus H / 2004 / Laccases: structure, reactions, distribution / Micron 35 : 93 ~ 96
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis Spores
Journal of Microbiology and Biotechnology
2019 .01
-
Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications
Journal of Microbiology and Biotechnology
2019 .01
-
A novel nonsense mutation in NPR2 gene causing Acromesomelic dysplasia, type Maroteaux in a consanguineous family in Southern Punjab (Pakistan)
Genes & Genomics
2020 .01
-
사상균인 Aspergillus nidulans의 무성포자 발아와 당의 역할
한국균학회지
2018 .01
-
An Approach for Lactulose Production Using the CotX-Mediated Spore-Displayed β-Galactosidase as a Biocatalyst
Journal of Microbiology and Biotechnology
2016 .01
-
Protein composition analysis of bacterial spores before and after UV laser irradiation by 1-D Gel Electrophoresis and MALDI-TOF-MS
한국분석과학회 학술대회
2019 .11
-
Inactivation of Bacillus atrophaeus bacterial spores by laser irradiation
한국분석과학회 학술대회
2020 .11
-
Regional differences in protein intake and protein sources of Korean older adults and their association with metabolic syndrome using the 2016–2019 Korea National Health and Nutrition Examination Surveys: a cross-sectional study
대한지역사회영양학회지
2024 .06
-
Production of Cyanocarboxylic Acid by Acidovorax facilis 72W Nitrilase Displayed on the Spore Surface of Bacillus subtilis
Journal of Microbiology and Biotechnology
2019 .01
-
Heat Shock Proteins: A Review of the Molecular Chaperones for Plant Immunity
The Plant Pathology Journal
2015 .12
-
Optimization for Scenedesmus obliquus Cultivation: the Effects of Temperature, Light Intensity and pH on Growth and Biochemical Composition
한국미생물·생명공학회지
2019 .01
-
Low-Cost Cultivation and Sporulation of Alkaliphilic Bacillus sp. Strain AK13 for Self-Healing Concrete
Journal of Microbiology and Biotechnology
2019 .01
-
Cryo-electron microscopy research at the institute of membrane proteins: current status and future prospects
Biodesign
2020 .01
-
Strategies in Protein Immobilization on a Gold Surface
Applied Science and Convergence Technology
2015 .01
-
A review of recent evidence of dietary protein intake and health
Nutrition Research and Practice
2022 .05
-
A review of recent evidence of dietary protein intake and health
Nutrition Research and Practice
2022 .05
-
Standardization of protein concentration of brazzein, a tryptophan-deficient sweet protein by relative protein quantification using diverse methods
한국분석과학회 학술대회
2015 .11
-
Structure Optimization of the Trp-Cage Protein in the Three-Dimensional Off-Lattice AB Protein Model
새물리
2022 .12
-
Efficient extraction of intact proteins from polyacrylamide gel by using ammonium sulfate
한국분석과학회 학술대회
2021 .05
-
Virus-like Protein Cage Nanoparticle Engineering for Effective Diagnosis & Therapy
한국진공학회 학술발표회초록집
2018 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0