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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제10호
- 발행연도
- 2015.1
- 수록면
- 1,709 - 1,713 (5page)
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초록· 키워드
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Sepiapterin is a precursor for the synthesis of tetrahydrobiopterin (BH4), which is a wellknown cofactor for aromatic amino acid hydroxylation and nitric oxide synthesis in higher mammals. In this study, a recombinant Escherichia coli BL21(DE3) strain harboring cyanobacterial guanosine 5’-triphosphate cyclohydrolase 1 (GCH1) and human 6- pyruvoyltetrahydropterin synthase (PTPS) genes was constructed to produce sepiapterin. The optimum conditions for T7 promoter–driven expression of GCH1 and PTPS were 30°C and 0.1 mM isopropyl-β-D-thioglucopyranoside (IPTG). The maximum sepiapterin concentration of 88.1 ± 2.4 mg/l was obtained in a batch cultivation of the recombinant E. coli, corresponding to an 18-fold increase in sepiapterin production compared with the control condition (37°C and 1 mM IPTG).
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참고문헌 (21)
참고문헌 신청
Barford PA / 1984 / Tetrahydrobiopterin metabolism in the temporal lobe of patients dying with senile dementia of Alzheimer type / J. Neurol. Neurosurg. Psychiatry 47 : 736 ~
Cho YR / 2011 / Sepiapterin inhibits cell proliferation and migration of ovarian cancer cells via down-regulation of p70S6K-dependent VEGFR-2 expression / Oncol. Rep 26 : 861
Curtius HC / 1982 / Tetrahydrobiopterin: efficacy in endogenous depression and Parkinson’s disease / J. Neural Transm 55 : 301 ~ 308
Frye RE / 2010 / Tetrahydrobiopterin as a novel therapeutic intervention for autism / Neurotherapeutics 7 : 241 ~ 249
Kaufman S. / 1958 / A new cofactor required for the enzymatic conversion of phenylalanine to tyrosine / J. Biol. Chem 230 : 931 ~ 939
Cho YR / 2011 / Sepiapterin inhibits cell proliferation and migration of ovarian cancer cells via down-regulation of p70S6K-dependent VEGFR-2 expression / Oncol. Rep 26 : 861
Curtius HC / 1982 / Tetrahydrobiopterin: efficacy in endogenous depression and Parkinson’s disease / J. Neural Transm 55 : 301 ~ 308
Frye RE / 2010 / Tetrahydrobiopterin as a novel therapeutic intervention for autism / Neurotherapeutics 7 : 241 ~ 249
Kaufman S. / 1958 / A new cofactor required for the enzymatic conversion of phenylalanine to tyrosine / J. Biol. Chem 230 : 931 ~ 939
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