지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제8호
- 발행연도
- 2013.1
- 수록면
- 1,107 - 1,115 (9page)
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초록· 키워드
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The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex quinquefasciatus. It is also known that Cyt1Aa, which is highly lipophilic, synergizes Cry11Aa by functioning as a surrogate membrane-bound receptor for the latter protein. Little is known, however, about whether Cyt1Aa can interact similarly with other Cry proteins not primarily mosquitocidal; for example, Cry2Aa, which is active against lepidopteran larvae, but essentially inactive or has very low toxicity to mosquito larvae. Here we demonstrate by ligand binding and enzyme-linked immunosorbent assays that Cyt1Aa and Cry2Aa form intermolecular complexes in vitro, and in addition show that Cyt1Aa facilitates binding of Cry2Aa throughout the midgut of C. quinquefasciatus larvae. As Cry2Aa and Cry11Aa share structural similarity in domain II, the interaction between Cyt1Aa and Cry2Aa could be a result of a similar mechanism previously proposed for Cry11Aa and Cyt1Aa. Finally, despite the observed interaction between Cry2Aa and Cyt1Aa, only a 2-fold enhancement in toxicity resulted against C. quinquefasciatus. Regardless, our results suggest that Cry2Aa could be a useful component of mosquitocidal endotoxin complements being developed for recombinant strains of B. thuringiensis subsp. israelensis and B. sphaericus aimed at improving the efficacy of commercial products and avoiding resistance.
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참고문헌 (22)
참고문헌 신청
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Bravo A / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 15 : 423 ~ 435
Butko P. / 2003 / Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypothesis / Appl. Environ. Microbiol 69 : 2415 ~ 2422
Crickmore N / 1995 / Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis / FEMS Microbiol. Lett 131
Diaz-Mendoza M / 2012 / A 54-kDa protein encoded by pBtoxis is required for parasporal body structural integrity in Bacillus thuringiensis subsp. israelensis / J. Bacteriol 1
Bravo A / 2007 / Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control / Toxicon 15 : 423 ~ 435
Butko P. / 2003 / Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypothesis / Appl. Environ. Microbiol 69 : 2415 ~ 2422
Crickmore N / 1995 / Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis / FEMS Microbiol. Lett 131
Diaz-Mendoza M / 2012 / A 54-kDa protein encoded by pBtoxis is required for parasporal body structural integrity in Bacillus thuringiensis subsp. israelensis / J. Bacteriol 1
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