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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제8호
- 발행연도
- 2013.1
- 수록면
- 1,084 - 1,091 (8page)
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초록· 키워드
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In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First,wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett–Burman experimental design. Subsequently, an optimal medium was obtained using the Box–Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4·7H2O, 0.1 g/l of MgSO4·7H2O, 0.1 g/l of MnSO4·2H2O, 0.1 g/l of ZnSO4·7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice.
With this optimal medium, the milk-clotting enzyme production was remarkably enhanced.
The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett–Burman design and Box–Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.
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참고문헌 (23)
참고문헌 신청
Ageitos JM / 2007 / Purification and characterization of a milk-clotting protease from Bacillus licheniformis strain USC13 / J. Appl. Microbiol 103 : 2205 ~ 2213
Ajay P / 2010 / Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-statefermentation / Bioresour. Technol 101 : 7563 ~ 7569
Aravindan R / 2009 / Statistical experimental design for evaluation of medium components for lipase production by Rhizopus arrhizus M T CC 2233 / LWT-Food Sci. Technol 42 : 9
Arima K / 1970 / Milk-clotting enzyme from Mucor pusillus var / Lindt. Methods Enzymol 19 : 446 ~ 459
Belur, Prasanna D. / 2012 / Optimization of Culture Medium for Novel Cell-Associated Tannase Production from Bacillus massiliensis Using Response Surface Methodology / Journa
Ajay P / 2010 / Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-statefermentation / Bioresour. Technol 101 : 7563 ~ 7569
Aravindan R / 2009 / Statistical experimental design for evaluation of medium components for lipase production by Rhizopus arrhizus M T CC 2233 / LWT-Food Sci. Technol 42 : 9
Arima K / 1970 / Milk-clotting enzyme from Mucor pusillus var / Lindt. Methods Enzymol 19 : 446 ~ 459
Belur, Prasanna D. / 2012 / Optimization of Culture Medium for Novel Cell-Associated Tannase Production from Bacillus massiliensis Using Response Surface Methodology / Journa
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