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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제9호
- 발행연도
- 2012.1
- 수록면
- 1,245 - 1,252 (8page)
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Acinetobacter venetians V28 was isolated from the intestine of righteye flounder, Poecilopsetta plinthus caught in Vietnam seawater, and the esterase gene was cloned using a shotgun method. The amino acid sequence deduced from the nucleotide sequence (1,017 bp) corresponded to a protein of 338 amino acid residues with a molecular weight of 37,186. The esterase had 87% and 72% identities with the lipases of A. junii SH205 and A. calcoaceticus RUH2202, respectively. The esterase contained a putative leader sequence, as well as the conserved catalytic triad (Ser, His, Asp), consensus pentapeptide GXSXG, and oxyanion hole sequence (HG). The protein from the strain V28 was produced in both a soluble and an insoluble form when the Escherichia coli cells harboring the gene were cultured at 18oC. The maximal activity of the purified enzyme was observed at a temperature of 40oC and pH 9.0using p-NP-caprylate as substrate; however, relative activity still reached to 70% even at 5oC with an activation energy of 3.36 kcal/mol, which indicated that it was a cold-adapted enzyme. The enzyme was a nonmetalloprotein and was active against p-nitrophenyl esters of C4,C8, and C14. Remarkably, this enzyme retained much of its activity in the presence of commercial detergents and organic solvents. This cold-adapted esterase will be applicable as catalysts for reaction in the presence of organic solvents and detergents.
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참고문헌 (23)
참고문헌 신청
Alquati, C. / 2002 / The cold-adapted lipase of Pseudomonas fragi: Heterologous expression, biochemical characterization and molecular modeling / Eur. J. Biochem 269 : 3321 ~
Bornscheuer, U. T. / 2002 / Microbial carboxylesterases: Classification, properties and applications in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Breuil, C. / 1974 / Partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (Acinetobacter O16) / Can. J. Microbiol 21 : 434 ~ 441
Chen, S. / 1998 / Production of an alkaline lipase by Acinetobacter radioresistens / Biotechnol. Bioeng 62 : 311 ~ 316
Choo, D. W. / 1998 / A cold-adapted lipase of an Alaskan psychrotroph,Pseudomonas sp. strain B11-1: Gene cloning and enzymepurification and characterization / Appl. Environ.
Bornscheuer, U. T. / 2002 / Microbial carboxylesterases: Classification, properties and applications in biocatalysis / FEMS Microbiol. Rev 26 : 73 ~ 81
Breuil, C. / 1974 / Partial purification and characterization of the lipase of a facultatively psychrophilic bacterium (Acinetobacter O16) / Can. J. Microbiol 21 : 434 ~ 441
Chen, S. / 1998 / Production of an alkaline lipase by Acinetobacter radioresistens / Biotechnol. Bioeng 62 : 311 ~ 316
Choo, D. W. / 1998 / A cold-adapted lipase of an Alaskan psychrotroph,Pseudomonas sp. strain B11-1: Gene cloning and enzymepurification and characterization / Appl. Environ.
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