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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제7호
- 발행연도
- 2012.1
- 수록면
- 990 - 999 (10page)
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초록· 키워드
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The microbial biosynthesis of fatty acid of lipid metabolism,which can be used as precursors for the production of fuels of chemicals from renewable carbon sources, has attracted significant attention in recent years. The regulation of fatty acid biosynthesis pathways has been mainly studied in a model prokaryote, Escherichia coli.
During the recent period, global regulation of fatty acid metabolic pathways has been demonstrated in another model prokaryote, Bacillus subtilis, as well as in Streptococcus pneumonia. The goal of this study was to increase the production of long-chain fatty acids by developing recombinant E. coli strains that were improved by an elongation cycle of fatty acid synthesis (FAS). The fabB,fabG, fabZ, and fabI genes, all homologous of E. coli, were induced to improve the enzymatic activities for the purpose of overexpressing components of the elongation cycle in the FAS pathway through metabolic engineering.
The β-oxoacyl-ACP synthase enzyme catalyzed the addition of acyl-ACP to malonyl-ACP to generate β-oxoacyl-ACP. The enzyme encoded by the fabG gene converted β-oxoacyl-ACP to β-hydroxyacyl-ACP, the fabZ catalyzed the dehydration of β-3-hydroxyacyl-ACP to trans-2-acyl-ACP, and the fabI gene converted trans-2-acyl-ACP to acyl-ACP for long-chain fatty acids. In vivo productivity of total lipids and fatty acids was analyzed to confirm the changes and effects of the inserted genes in E.
coli. As a result, lipid was increased 2.16-fold higher and hexadecanoic acid was produced 2.77-fold higher in E.
coli JES1030, one of the developed recombinants through this study, than those from the wild-type E. coli.
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참고문헌 (22)
참고문헌 신청
Atsumi, S. / 2008 / Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels / Nature Lett 451 : 86 ~ 89
Atsumi, S. / 2010 / Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/ alcohol dehydrogenase genes / Appl. Microbi
Bligh, E. G. / 1959 / A rapid method of total lipid extraction and purification / Can. J. Biochem. Physiol 37 : 911 ~ 917
Campbell, J. W. / 2001 / Bacterial fatty acid biosynthesis: Targets for antibacterial drug discovery / Annu. Rev. Microbiol 55 : 305 ~ 332
David, I. / 2010 / Molecular dynamics simulations of β-ketoacyl-, β-hydroxyacyl-, and trans- 2-enoyl-acyl carrier proteins of Escherichia coli / Biochemistry 49 : 2860 ~ 28
Atsumi, S. / 2010 / Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/ alcohol dehydrogenase genes / Appl. Microbi
Bligh, E. G. / 1959 / A rapid method of total lipid extraction and purification / Can. J. Biochem. Physiol 37 : 911 ~ 917
Campbell, J. W. / 2001 / Bacterial fatty acid biosynthesis: Targets for antibacterial drug discovery / Annu. Rev. Microbiol 55 : 305 ~ 332
David, I. / 2010 / Molecular dynamics simulations of β-ketoacyl-, β-hydroxyacyl-, and trans- 2-enoyl-acyl carrier proteins of Escherichia coli / Biochemistry 49 : 2860 ~ 28
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