지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제12호
- 발행연도
- 2011.1
- 수록면
- 1,280 - 1,286 (7page)
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초록· 키워드
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A quantitative, real-time PCR method was developed to enumerate Lactobacillus plantarum IWBT B 188 during the malolactic fermentation (MLF) in Grauburgunder wine. The qRT-PCR was strain-specific, as it was based on primers targeting a plasmid DNA sequence, or it was L.
plantarum-specific, as it targeted a chromosomally located plantaricin gene sequence. Two 50 l wine fermentations were prepared. One was inoculated with 15 g/hl Saccharomyces cerevisiae, followed by L. plantarum IWBT B 188 at 3.6 ×10^6 CFU/ml, whereas the other was not inoculated (control). Viable cell counts were performed for up to 25days on MRS agar, and the same cells were enumerated by qRT-PCR with both the plasmid or chromosomally encoded gene primers. The L. plantarum strain survived under the harsh conditions in the wine fermentation at levels above 10^5/ml for approx. 10 days, after which cell numbers decreased to levels of 10³ CFU/ml at day 25, and to below the detection limit after day 25. In the control, no lactic acid bacteria could be detected throughout the fermentation, with the exception of two sampling points where ca. 1 × 10² CFU/ml was detected. The minimum detection level for quantitative PCR in this study was 1 ×10² to 1 × 10³ CFU/ml. The qRT-PCR results determined generally overestimated the plate count results by about 1log unit, probably as a result of the presence of DNA from dead cells. Overall, qRT-PCR appeared to be well suited for specifically enumerating Lactobacillus plantarum starter cultures in the MLF in wine.
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참고문헌 (21)
참고문헌 신청
[학술지(정기간행
Birnboim, H. C / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Bjorkroth, J / 1996 / Evaluation of Lactobacillus sakei contamination in vacuum-packaged sliced cooked meat products by ribotyping / J. Food Prot 59 : 398 ~ 401
Cho, G.-S / 2010 / Genetic analysis of the plantaricin EFI locus of Lactobacillus plantarum PCS20 reveals an unusual plantaricin E gene sequence as a result of mutation / Int
Cho, G.-S / 2010 / Investigation into the Potential of Bacteriocinogenic Lactobacillus plantarum BFE 5092 for Biopreservation of Raw Turkey Meat / Probiotics and Antimicrobia
Birnboim, H. C / 1979 / A rapid alkaline extraction procedure for screening recombinant plasmid DNA / Nucleic Acids Res 7 : 1513 ~ 1523
Bjorkroth, J / 1996 / Evaluation of Lactobacillus sakei contamination in vacuum-packaged sliced cooked meat products by ribotyping / J. Food Prot 59 : 398 ~ 401
Cho, G.-S / 2010 / Genetic analysis of the plantaricin EFI locus of Lactobacillus plantarum PCS20 reveals an unusual plantaricin E gene sequence as a result of mutation / Int
Cho, G.-S / 2010 / Investigation into the Potential of Bacteriocinogenic Lactobacillus plantarum BFE 5092 for Biopreservation of Raw Turkey Meat / Probiotics and Antimicrobia
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