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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제12호
- 발행연도
- 2011.1
- 수록면
- 1,336 - 1,344 (9page)
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This study details and examines a novel ligation-mediated polymerase chain reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4-base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s) determine a subset of the genomic restriction fragments, which are amplified by PCR. The method permits the differentiation of bacterial species strains on the basis of the different DNA band patterns obtained after electrophoresis in polyacrylamide gels stained with ethidium bromide and visualized in UV light. The usefulness of the LM-PCR/Shifter method for genotyping is analyzed by a comparison with the restriction endonuclease analysis of chromosomal DNA by the pulsed-field gel electrophoresis (REA-PFGE) and PCR melting profile (PCR MP)methods for isolates of clinical origin. The clustering of the LM-PCR/Shifter fingerprinting data matched those of the REA-PFGE and PCR MP methods. We found that the LM-PCR/Shifter is rapid, and offers good discriminatory power and excellent reproducibility, making it a method that may be effectively applied in epidemiological studies.
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참고문헌 (19)
참고문헌 신청
Fevre, C / 2005 / Six groups of the OXY β-lactamase evolved over millions of years in Klebsiella oxytoca / Antimicrob. Agents Chemother 49 : 3453 ~ 3462
Goering, R. V / 2010 / Pulsed field gel electrophoresis: A review of application and interpretation in the molecular epidemiology of infectious disease / Infect. Genet. Evol
Krawczyk, B / 2003 / Evaluation and comparison of random amplification of polymorphic DNA, pulsed-field gel electrophoresis and ADSRRS-fingerprinting for typing Serratia marc
Krawczyk, B / 2003 / Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites (ADSRRS fingerprinting) for typing strains of van
Krawczyk, B / 2005 / Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: Control of an outbreak using a new ADSRRS technique / Pol. J. Microb
Goering, R. V / 2010 / Pulsed field gel electrophoresis: A review of application and interpretation in the molecular epidemiology of infectious disease / Infect. Genet. Evol
Krawczyk, B / 2003 / Evaluation and comparison of random amplification of polymorphic DNA, pulsed-field gel electrophoresis and ADSRRS-fingerprinting for typing Serratia marc
Krawczyk, B / 2003 / Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites (ADSRRS fingerprinting) for typing strains of van
Krawczyk, B / 2005 / Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: Control of an outbreak using a new ADSRRS technique / Pol. J. Microb
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