개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제12호
- 발행연도
- 2011.1
- 수록면
- 1,203 - 1,210 (8page)
이용수
초록· 키워드
오류제보하기
Function-driven metagenomic analysis is a powerful approach to screening for novel biocatalysts. In this study,we investigated lipolytic enzymes selected from an alluvial soil metagenomic library, and identified two novel esterases, EstDL26 and EstDL136. EstDL26 and EstDL136reactivated chloramphenicol from its acetyl derivates by counteracting the chloramphenicol acetyltransferase (CAT) activity in Escherichia coli. These two enzymes showed only 27% identity in amino acid sequence to each other; however both preferentially hydrolyzed short-chain p-nitrophenyl esters (≤C_5) and showed mesophilic properties.
In vitro, EstDL136 catalyzed the deacetylation of 1- and 3-acetyl and 1,3-diacetyl derivates; in contrast, EstDL26was not capable of the deacetylation at C₁, indicating a potential regioselectivity. EstDL26 and EstDL136 were similar to microbial hormone-sensitive lipase (HSL), and since chloramphenicol acetate esterase (CAE) activity was detected from two other soil esterases in the HSL family, this suggests a distribution of CAE among the soil microorganisms.
The isolation and characterization of EstDL26 and EstDL136 in this study may be helpful in understanding the diversity of CAE enzymes and their potential role in releasing active chloramphenicol in the producing bacteria.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Alexander, N. J / 2011 / The genetic basis for 3-ADON and 15-ADON trichothecene chemotypes in Fusarium / Fungal Genet. Biol 48 : 485 ~ 495
Amann, R. I / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev 59 : 143 ~ 169
Arpigny, J. L / 1999 / Bacterial lipolytic/enzymes: Classification and properties / Biochem. J 343 : 177 ~ 183
Bornscheuer, U. T / 2002 / Microbial carboxyl esterases: Classification, properties and application in biocatalysis / FEMS. Microbiol. Rev 26 : 73 ~ 81
Cundliffe, E / 1989 / How antibiotic-producing organisms avoid suicide / Annu. Rev. Microbiol 43 : 207 ~ 233
Amann, R. I / 1995 / Phylogenetic identification and in situ detection of individual microbial cells without cultivation / Microbiol. Rev 59 : 143 ~ 169
Arpigny, J. L / 1999 / Bacterial lipolytic/enzymes: Classification and properties / Biochem. J 343 : 177 ~ 183
Bornscheuer, U. T / 2002 / Microbial carboxyl esterases: Classification, properties and application in biocatalysis / FEMS. Microbiol. Rev 26 : 73 ~ 81
Cundliffe, E / 1989 / How antibiotic-producing organisms avoid suicide / Annu. Rev. Microbiol 43 : 207 ~ 233
이 논문의 저자 정보
최근 본 자료
댓글(0)
0