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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 281 - 286 (6page)
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초록· 키워드
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A Pseudomonas fluorescens strain was isolated and found to show antagonistic activity against phytopathogenic fungi and to possess a gene responsible for production of antibiotic 2,4-diacetylphloroglucinol. For the extension of biocontrol range, a gene for an Androctonus australis Hector insect toxin 1 (AaHIT1), one of the most known toxic insect-selective peptides, was designed and synthesized according to the preferred codon usage of Pseudomonas fluorescens, cloned, and transformed into the strain by pSUP106 vector, a broad-host-range plasmid. Bioassays indicated that the engineered strain was able to produce AaHIT1 with insecticidal activity, and at the same time retain the activity against plant pathogen. The experiments for nonplanted soil and rhizosphere colonization showed that,similar to the population of the wild-type strain, that of the engineered strain remained relatively constant in the first 10 days, and the subsequent 50 days, suggesting that AaHIT1 expression in the bacterial cell does not substantially impair its long-term colonization. It is first reported that a Pseudomonas fluorescens strain expressing an active scorpion neurotoxin has dual activity against phytopathogenic fungi and insects, making at attractive for agronomic applications.
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참고문헌 (22)
참고문헌 신청
Ausubel, F. M / 1995 / Short Protocols in Molecular Biology / John Wiley & Sons, Inc
Bangera, M. G / 1999 / Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescen
Calogero, R. A / 1988 / Selection of the mRNA translation initiation region by Escherichia coli ribosomes / Proc. Natl. Acad. Sci. U.S.A : 6427 ~ 6431
Cory, J. S / 1994 / Field trial of a genetically improved baculovirus insecticide / Nature 370 : 138 ~ 140
Darbon, H / 1991 / Two dimensional 1H nuclear magnetic resonance study of AaH IT, an anti-insect toxin from Androctonus australis Hector. Sequential resonance assignments and
Bangera, M. G / 1999 / Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescen
Calogero, R. A / 1988 / Selection of the mRNA translation initiation region by Escherichia coli ribosomes / Proc. Natl. Acad. Sci. U.S.A : 6427 ~ 6431
Cory, J. S / 1994 / Field trial of a genetically improved baculovirus insecticide / Nature 370 : 138 ~ 140
Darbon, H / 1991 / Two dimensional 1H nuclear magnetic resonance study of AaH IT, an anti-insect toxin from Androctonus australis Hector. Sequential resonance assignments and
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