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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 415 - 424 (10page)
이용수
초록· 키워드
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The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A nonpolar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to create the ΔluxS mutant strain ML03J. Phenotypic characterization of wild-type and mutant strains demonstrated that ML03J had no obvious growth or metabolic defects on 0.2%glucose LB medium, produced a functionally defective flagellum, and could not utilize sorbose; the biological significance of sorbose utilization is unknown. Omicsbased analysis revealed the involvement of LuxS in the transcriptional activation of several flagella/chemotaxisrelated genes (flhD; fliA, C, D, S, Z; and cheA, Y, Z),repression of glutamate-dependent acid resistance genes (gadAB), and expression of virulence factors including Shiga toxin, hemolysin, and SepD within the LEE pathogenicity island.
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참고문헌 (23)
참고문헌 신청
Balaban, N / 1995 / Autocrine regulation of toxin synthesis by Staphylococcus aureus / Proc. Natl. Acad. Sci. U.S.A : 1619 ~ 1623
Bansal, T / 2008 / Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2 / Appl. Microbiol. Biotechnol 78 : 811 ~ 819
Bhagwat, A. A / 2004 / Comparative analysis of transcriptional regulatory elements of glutamate-dependent acid-resistance systems of Shigella flexneri and Escherichia coli O1
Chen, X / 2002 / Structural identification of a bacterial quorum-sensing signal containing boron / Nature 415 : 545 ~ 549
Cherepanov, P. P / 1995 / Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant / Gene
Bansal, T / 2008 / Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2 / Appl. Microbiol. Biotechnol 78 : 811 ~ 819
Bhagwat, A. A / 2004 / Comparative analysis of transcriptional regulatory elements of glutamate-dependent acid-resistance systems of Shigella flexneri and Escherichia coli O1
Chen, X / 2002 / Structural identification of a bacterial quorum-sensing signal containing boron / Nature 415 : 545 ~ 549
Cherepanov, P. P / 1995 / Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant / Gene
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