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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제3호
- 발행연도
- 2014.1
- 수록면
- 427 - 430 (4page)
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초록· 키워드
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We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona,Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eightmonoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus3G11 showed the greatest reactivity. The detection limit of the kit was 6.3 × 106 CFU per test.
Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligaseICA, and 27 non-vanA vancomycin-resistant isolates were negative. These results wereconsistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-useimmunological assay for detecting VanA-producing VRE in clinical laboratories.
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참고문헌 (16)
참고문헌 신청
Bergmans HEN / 1981 / Transformation in Escherichia coli: stages in the process / J. Bacteriol 146 : 564 ~ 570
Bourdon N / 2010 / Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay / Diagn. Microbiol. Infect. Dis 67 : 291 ~ 293
Cheong HJ / 2002 / Clinical features of VRE infection, microbiologic characteristics and genetic relatedness of VRE isolates from six university hospitals / Korean J. Infect.
Clark NC / 2005 / Comparison of Tn1546-like elements in vancomycin-resistant Staphylococcus aureus isolates from Michigan and Pennsylvania / Antimicrob. Agents Chemother 49 :
Conceicao N / 2011 / Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study / Rev. Soc. Bras. Med. Trop 44
Bourdon N / 2010 / Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay / Diagn. Microbiol. Infect. Dis 67 : 291 ~ 293
Cheong HJ / 2002 / Clinical features of VRE infection, microbiologic characteristics and genetic relatedness of VRE isolates from six university hospitals / Korean J. Infect.
Clark NC / 2005 / Comparison of Tn1546-like elements in vancomycin-resistant Staphylococcus aureus isolates from Michigan and Pennsylvania / Antimicrob. Agents Chemother 49 :
Conceicao N / 2011 / Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study / Rev. Soc. Bras. Med. Trop 44
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