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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제6호
- 발행연도
- 2012.1
- 수록면
- 736 - 741 (6page)
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초록· 키워드
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In this study, we analyzed the oxidative stress response of wblA (whiB-like gene A, SCO3579), which was previously shown to be a global antibiotic down-regulator in Streptomyces coelicolor. Ever since a WblA ortholog named WhcA in Corynebacterium glutamicum was found to play a negative role in the oxidative stress response, S. coelicolor wblA has been proposed to have a similar effect. A wblA-deletion mutant exhibited a less sensitive response to oxidative stress induced by diamide present in solid plate culture.
Using real-time RT-PCR analysis, we also compared the transcription levels of oxidative stress-related genes,including sodF, sodF2, sodN, trxB, and trxB2, between S.
coelicolor wild type and a wblA-deletion mutant in the presence or absence of oxidative stress. Target genes were expressed higher in the wblA-deletion mutant compared with wild type, both in the absence and presence of oxidative stress. Moreover, expression of these target genes in S.
coelicolor wild type was stimulated only in the presence of oxidative stress, suggesting that WblA plays a negative role in the oxidative stress response of S. coelicolor, similar to that of C. glutamicum WhcA, through the transcriptional regulation of oxidative stress-related genes.
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참고문헌 (20)
참고문헌 신청
Arner, E. S. / 2000 / Physiological functions of thioredoxin and thioredoxin reductase / Eur. J. Biochem 267 : 6102 ~ 6109
Choi, W. W. / 2009 / The whcA gene plays a negative role in oxidative stress response of Corynebacterium glutamicum / FEMS MICROBIOLOGY LETTERS 290 (0) : 32 ~ 38
Chung, H. J. / 1999 / Duplicate genes for Fe-containing superoxide dismutasein Streptomyces coelicolor A3(2) / Gene 231 : 87 ~ 93
Crack, J. C. / 2009 / Characterization of [4Fe-4S]-containing and cluster-free forms of Streptomyces WhiD / Biochemistry 48 : 12252 ~ 12264
Crack, J. C. / 2011 / Mechanistic insight into the nitrosylation of the [4Fe-4S] cluster of WhiB-like protein / J. Am. Chem. Soc 133 : 1112 ~ 1121
Choi, W. W. / 2009 / The whcA gene plays a negative role in oxidative stress response of Corynebacterium glutamicum / FEMS MICROBIOLOGY LETTERS 290 (0) : 32 ~ 38
Chung, H. J. / 1999 / Duplicate genes for Fe-containing superoxide dismutasein Streptomyces coelicolor A3(2) / Gene 231 : 87 ~ 93
Crack, J. C. / 2009 / Characterization of [4Fe-4S]-containing and cluster-free forms of Streptomyces WhiD / Biochemistry 48 : 12252 ~ 12264
Crack, J. C. / 2011 / Mechanistic insight into the nitrosylation of the [4Fe-4S] cluster of WhiB-like protein / J. Am. Chem. Soc 133 : 1112 ~ 1121
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