지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제21권 제7호
- 발행연도
- 2011.1
- 수록면
- 719 - 727 (9page)
이용수
초록· 키워드
오류제보하기
Botulism is a neuroparalytic disease caused by Clostridium botulinum, which produces seven (A-G) antigenically diverse neurotoxins (BoNTs). BoNTs are the most poisonous substances known to humans, with a median lethal dose (LD50) of approximately 1 ng/kg of body weight. Owing to their extreme potency and lethality, they have the potential to be used as a bioterrorism agent. The mouse bioassay is the gold standard for the detection of botulinum neurotoxins; however, it requires at least 3-4 days for completion. Attempts have been made to develop an ELISA-based detection system, which is potentially an easier and more rapid method of botulinum neurotoxin detection. The present study was designed using a synthetic gene approach. The synthetic gene encoding the catalytic domain of BoNT serotype B from amino acids 1-450 was constructed with PCR overlapping primers (BoNT/B LC),cloned in a pQE30 UA vector, and expressed in an E. coli M15 host system. Recombinant protein production was optimized at 0.5 mM IPTG final concentration, 4 h post induction, resulting in a maximum yield of recombinant proteins. The immunogenic nature of the recombinant BoNT/B LC protein was evaluated by ELISA. Antibodies were raised in BALB/c mice using various adjuvants. A significant rise in antibody titer (p<0.05) was observed in the Alum group, followed by the Titermax Classic group,Freund’s adjuvant, and the Titermax Gold group. These developed high-titer antibodies may prove useful for the detection of botulinum neurotoxins in food and clinical samples.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Agarwal, A. K / 2004 / Food-borne botulism / J. Assoc. Physicians India 52 : 677 ~ 678
Akdeniz, H. / 2007 / An outbreak of botulism in a family in Eastern Anatolia associated with eating suzme yoghurt buried under soil / Scand. J. Infect. Dis 39 : 108 ~ 114
Arnon, S. S / 2001 / Botulinum toxin as a biological weapon: Medical and public health management / J. Am. Med. Assoc 285 : 1059 ~ 1070
Barash, J. R / 2005 / First case of infant botulism caused by Clostridium baratii type F in California / J. Clin. Microbiol 43 : 4280 ~ 4282
Binz, T. / 1994 / Proteolysis of SNAP- 25 by types E and A botulinal neurotoxins / J. Biol. Chem 269 : 1617 ~ 1620
Akdeniz, H. / 2007 / An outbreak of botulism in a family in Eastern Anatolia associated with eating suzme yoghurt buried under soil / Scand. J. Infect. Dis 39 : 108 ~ 114
Arnon, S. S / 2001 / Botulinum toxin as a biological weapon: Medical and public health management / J. Am. Med. Assoc 285 : 1059 ~ 1070
Barash, J. R / 2005 / First case of infant botulism caused by Clostridium baratii type F in California / J. Clin. Microbiol 43 : 4280 ~ 4282
Binz, T. / 1994 / Proteolysis of SNAP- 25 by types E and A botulinal neurotoxins / J. Biol. Chem 269 : 1617 ~ 1620
이 논문의 저자 정보
최근 본 자료
댓글(0)
0